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Micología

Líneas de investigación

Content with Investigacion Entomología Médica .

The Laboratory of Medical Entomology (LME) develops an intense reference and research activity, focused on the field of disease vectors of interest in Public Health. The LME has an insectary where biological cycles of insect vectors are currently maintained, allowing the performance, among others, of vector competence and xenodiagnostic studies. The LME supports the national health system by offering techniques available in the portfolio of services for the taxonomic identification of arthropods of health interest. In addition, it performs entomological surveillance of outbreaks, supporting Surveillance Plans. In particular, the LME plays a leading role in the Entomological Surveillance Plan for Leishmaniasis in the Community of Madrid. On the other hand, the LME offers scientific advice to the CCAES (Centro de Coordinación de Alertas y Emergencias Sanitarias, Ministerio de Sanidad, Consumo y Bienestar Social), and participates in the elaboration of reports and rapid risk assessments.


 

The main research lines of the Laboratory of Medical Entomology are:

1. Maintenance of insect vector colonies: phlebotomine sand flies (Phlebotomus perniciosus, Phlebotomus papatasi and Phlebotomus argentipes, vectors of Leishmania infantum, Leishmania major and Leishmania donovani, respectively), Culex and Aedes mosquitoes (vectors of various arboviruses) and Rhodnius prolixus (vector of Trypanosoma cruzi). 
2. Biology of disease vectors of public health interest: biology, vector competence, experimental infections. The CNM has a BSL3 safety laboratory to carry out vector competence studies with culicidae and phlebotomine sand flies.
3. Entomological sampling, infectivity of potential reservoirs of leishmaniasis.
4. Insecticides and repellents: evaluation of their efficacy. 
5. Characterization of saliva proteins of hematophagous Diptera: genomics, proteomics, biochemistry and gene editing. Study of salivary proteins as markers of bite exposure, virulence factors and/or vaccines.
6. Xenodiagnosis of leishmaniasis.
7. Molecular biology and taxonomy of phlebotomine sand flies. Molecular detection of Leishmania infantum in phlebotomine sand flies and characterization of Leishmania spp. Molecular identification of blood ingested by vectors.

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Alastruey-Izquierdo A, Alcazar-Fuoli L, Rivero-Menéndez O, Ayats J, Castro C, García-Rodríguez J, Goterris-Bonet L, Ibáñez-Martínez E, Linares-Sicilia MJ, Martin-Gomez MT, Martín-Mazuelos E, Pelaez T, Peman J, Rezusta A, Rojo S, Tejero R, Anza DV, Viñuelas J, Zapico MS, Cuenca-Estrella M; the FILPOP2 Project from GEMICOMED (SEIMC) and REIPI. Molecular Identification and Susceptibility Testing of Molds Isolated in a Prospective Surveillance of Triazole Resistance in Spain (FILPOP2 Study). Antimicrob Agents Chemother. 2018 Aug 27;62(9):e00358-18. doi: 10.1128/AAC.00358-18. PMID: 29941643; PMCID: PMC6125503.

Alastruey-Izquierdo A, Alcazar-Fuoli L, Rivero-Menéndez O, Ayats J, Castro C, García-Rodríguez J, Goterris-Bonet L, Ibáñez-Martínez E, Linares-Sicilia MJ, Martin-Gomez MT, Martín-Mazuelos E, Pelaez T, Peman J, Rezusta A, Rojo S, Tejero R, Anza DV, Viñuelas J, Zapico MS, Cuenca-Estrella M; the FILPOP2 Project from GEMICOMED (SEIMC) and REIPI. Molecular Identification and Susceptibility Testing of Molds Isolated in a Prospective Surveillance of Triazole Resistance in Spain (FILPOP2 Study). Antimicrob Agents Chemother. 2018 Aug 27;62(9):e00358-18. doi: 10.1128/AAC.00358-18. PMID: 29941643; PMCID: PMC6125503.

PUBMED DOI

Gonçalves SM, Lagrou K, Rodrigues CS, Campos CF, Bernal-Martínez L, Rodrigues F, Silvestre R, Alcazar-Fuoli L, Maertens JA, Cunha C, Carvalho A. Evaluation of Bronchoalveolar Lavage Fluid Cytokines as Biomarkers for Invasive Pulmonary Aspergillosis in At-Risk Patients. Front Microbiol. 2017 Nov 29;8:2362. doi:10.3389/fmicb.2017.02362. PMID: 29238334; PMCID: PMC5712575.

Gonçalves SM, Lagrou K, Rodrigues CS, Campos CF, Bernal-Martínez L, Rodrigues F, Silvestre R, Alcazar-Fuoli L, Maertens JA, Cunha C, Carvalho A. Evaluation of Bronchoalveolar Lavage Fluid Cytokines as Biomarkers for Invasive Pulmonary Aspergillosis in At-Risk Patients. Front Microbiol. 2017 Nov 29;8:2362. doi:10.3389/fmicb.2017.02362. PMID: 29238334; PMCID: PMC5712575.

PUBMED DOI

Alcazar-Fuoli L, Buitrago M, Gomez-Lopez A, Mellado E. An alternative host model of a mixed fungal infection by azole susceptible and resistant Aspergillus spp strains. Virulence. 2015;6(4):376-84. doi: 10.1080/21505594.2015.1025192. PMID: 26065322; PMCID: PMC4601236.

Alcazar-Fuoli L, Buitrago M, Gomez-Lopez A, Mellado E. An alternative host model of a mixed fungal infection by azole susceptible and resistant Aspergillus spp strains. Virulence. 2015;6(4):376-84. doi: 10.1080/21505594.2015.1025192. PMID: 26065322; PMCID: PMC4601236.

PUBMED DOI

Alcazar-Fuoli L, Cairns T, Lopez JF, Zonja B, Pérez S, Barceló D, Igarashi Y, Bowyer P, Bignell E. A modified recombineering protocol for the genetic manipulation of gene clusters in Aspergillus fumigatus. PLoS One. 2014 Nov 5;9(11):e111875. doi: 10.1371/journal.pone.0111875. PMID: 25372385; PMCID:PMC4221250.

Alcazar-Fuoli L, Cairns T, Lopez JF, Zonja B, Pérez S, Barceló D, Igarashi Y, Bowyer P, Bignell E. A modified recombineering protocol for the genetic manipulation of gene clusters in Aspergillus fumigatus. PLoS One. 2014 Nov 5;9(11):e111875. doi: 10.1371/journal.pone.0111875. PMID: 25372385; PMCID:PMC4221250.

PUBMED DOI

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List of staff

Información adicional

El Laboratorio de Referencia e Investigación en Micología del Centro Nacional de Microbiología del Instituto de Salud Carlos III es el laboratorio de referencia de Micología para toda España. Además, mantiene varias líneas de investigación, entre las que destacan: Identificación de levaduras y hongos filamentosos, estudio de los mecanismos de resistencia de los hongos a los antifúngicos, estandarización de las pruebas de sensibilidad a los antifúngicos de los hongos patógenos, correlación in vivo-in vitro con modelos experimentales y pacientes, epidemiología molecular de la infección fúngica nosocomial, determinación de niveles de antifúngicos y diagnóstico rápido de las micosis invasoras mediante técnicas de diagnóstico rápido como PCR en Tiempo Real. El grupo de Investigadores constituye un equipo multidisciplinar con capacidad de formar y producir conocimiento científico de calidad, en el área de micología médica. Durante los últimos años se han leído 5 tesis doctorales en el departamento y actualmente el laboratorio cuenta con tres becarios pre-doctorales y dos postdoctorales. Además, cada año acogemos 3-5 estudiantes que desarrollan sus proyectos fin de Master o Grado y rotantes de la especialidad de Microbiología Clínica de distinta procedencia Nacional. Todos ellos participan en un programa de formación que comprende sesiones bibliográficas, teóricas y reuniones científicas que les permiten adquirir una formación global de la micología.

Content with Investigacion Entomología Médica .