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Content with Investigacion Infecciones Bacterianas Transmitidas por Agua y Alimentos .

Legionella

Desde su creación hasta la actualidad, La Unidad de Legionella tiene como principal función dar apoyo científico-técnico a la Administración General del Estado, a las Comunidades Autónomas y al Sistema Nacional de Salud en el campo de la prevención y control de la legionelosis, así como llevar a cabo investigaciones científicas en el contexto de la legionelosis. Además, la Unidad de Legionella también actúa como Laboratorio de Referencia de España frente al European Centre for Disease Prevention and Control (ECDC), siendo miembro de la red europea de vigilancia de la legionelosis, “European Legionnaires’ Disease Surveillance Network (ELDSNet). Finalmente, la unidad también realiza una actividad docente, participando en cursos de formación especializada, así como en Máster Universitarios.

 

Principales líneas de investigación

Vigilancia microbiológica

Búsqueda de marcadores moleculares con capacidad de predecir el riesgo de una instalación de provocar legionelosis. Factores de virulencia de Legionella spp.

Estudio de la capacidad formadora de biofilms de Legionella spp. Colonización y dispersión.

Búsqueda de marcadores fenotípicos capaces de discriminar especies del Género Legionella; grupos y subgrupos de Legionella pneumophila.

Diferentes estructuras de biofilms en función de la cepa formadora de Legionella pneumophila. En verde la biomasa bacteriana, en rojo el exopolisacárido de la matriz extracelular.

Apoyo al Sistema Nacional de Salud de la Unidad de Legionella

 

La Unidad de Legionella tambien desarrolla actividades con el fin de proporcionar asistencia al sistema nacional de salud a traves de la oferta disponible en la cartera de servicios del CNM, así como a través de programas de vigilancia microbiológica.

Enfermedades bacterianas transmitidas por agua y alimentos

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Research projects

Content with Investigacion Infecciones Bacterianas Transmitidas por Agua y Alimentos .

1: Título del proyecto: Búsqueda de biomarcadores de patogenicidad en Legionella spp con interés predictivo de riesgo de infección.
Investigador principal: Fernando González Camacho
Entidad financiadora: ISCIII (AESI). Referencia:  MPY 341/22
Periodo: 01/01/2023 - 31/12/2025

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Sequence analysis of in vivo-expressed HIV-1 spliced RNAs reveals the usage of new and unusual splice sites by viruses of different subtypes

Vega Y, Delgado E, de la Barrera J, Carrera C, Zaballos Á, Cuesta I, Mariño A, Ocampo A, Miralles C, Pérez-Castro S, Álvarez H, López-Miragaya I, García-Bodas E, Díez-Fuertes F, Thomson MM. Sequence analysis of in vivo-expressed HIV-1 spliced RNAs reveals the usage of new and unusual splice sites by viruses of different subtypes. PLoS One. 2016; 11:e0158525.

PUBMED DOI

HIV-1 genetic diversity in recently diagnosed infections in Moscow: predominance of AFSU, frequent branching in clusters, and circulation of the Iberian subtype G variant.

Karamov E, Epremyan K, Siniavin A, Zhernov Y, Cuevas MT, Delgado E, Sánchez-Martínez M, Carrera C, Kornilaeva G, Turgiev A, Bacqué J, Pérez-Álvarez L, Thomson MM. HIV-1 genetic diversity in recently diagnosed infections in Moscow: predominance of AFSU, frequent branching in clusters, and circulation of the Iberian subtype G variant. AIDS Res Hum Retroviruses. 2018; 34:629-634.

PUBMED DOI

Bayesian phylogeographic analyses clarify the origin of the HIV-1 subtype A variant circulating in former Soviet Union's countries.

Díez-Fuertes F, Cabello M, Thomson MM. Bayesian phylogeographic analyses clarify the origin of the HIV-1 subtype A variant circulating in former Soviet Union's countries. Infect Genet Evol. 2015; 33:197-205.

PUBMED DOI

Alcazar-Fuoli L, Clavaud C, Lamarre C, Aimanianda V, Seidl-Seiboth V, Mellado E, Latgé JP. Functional analysis of the fungal/plant class chitinase family in Aspergillus fumigatus.

Alcazar-Fuoli L, Clavaud C, Lamarre C, Aimanianda V, Seidl-Seiboth V, Mellado E, Latgé JP. Functional analysis of the fungal/plant class chitinase family in Aspergillus fumigatus. Fungal Genet Biol. 2011 Apr;48(4):418-29. doi: 10.1016/j.fgb.2010.12.007. Epub 2010 Dec 22. PMID: 21184840.

PUBMED DOI

Impact of DARC rs12075 Variants on Liver Fibrosis Progression in Patients with Chronic Hepatitis C: A Retrospective Study.

Jiménez-Sousa MA (AC); Gómez-Moreno AZ; Pineda-Tenor D; et al. (1/9) Impact of DARC rs12075 Variants on Liver Fibrosis Progression in Patients with Chronic Hepatitis C: A Retrospective Study. Biomolecules 2019; 9(4).​

DBP rs16846876 and rs12512631 polymorphisms are associated with progression to AIDS naïve HIV-infected patients: a retrospective study.

Jiménez-Sousa MA (AC); Jiménez JL; Fernández-Rodríguez A; et al. (1/10). DBP rs16846876 and rs12512631 polymorphisms are associated with progression to AIDS naïve HIV-infected patients: a retrospective study. Journal of Biomedical Science. 2019; 23;26(1):83. doi: 10.1186/s12929-019-0577-y.

TRPM5 rs886277 Polymorphism Predicts Hepatic Fibrosis Progression in Non-Cirrhotic HCV-Infected Patients.Journal of Clinical Medicine.

Resino S; Fernández-Rodríguez A; Pineda-Tenor D; et al; Jiménez-Sousa MA. (11/11). 2021. TRPM5 rs886277 Polymorphism Predicts Hepatic Fibrosis Progression in Non-Cirrhotic HCV-Infected Patients.Journal of Clinical Medicine. 10-3, pp.483. ISSN 2077-0383. https://doi.org/10.3390/jcm10030483.

Plasma metabolomic fingerprint of advanced cirrhosis stages among HIV/HCV-coinfected and HCV-monoinfected patients

Salguero, Sergio; Rojo, David; Berenguer, Juan; et al; Jimenez-Sousa, Maria A. (AC) (15/15). 2020. Plasma metabolomic fingerprint of advanced cirrhosis stages among HIV/HCV-coinfected and HCV-monoinfected patients LIVER INTERNATIONAL. 40-9, pp.2215-2227. ISSN 1478-3223. https://doi.org/10.1111/liv.14580 3

Content with Investigacion Infecciones Bacterianas Transmitidas por Agua y Alimentos .

List of staff

Additional Information

Our group is interested in the mechanisms of generation of hematopoietic cells throughout ontogeny and the influence that the first hematopoietic cells, belonging to the innate/pseudoinnate immune system, exert on the adaptive immune system in the adult individual. Our lines of research include the analysis of pseudoinnate lymphoid populations, which connect the innate and adaptive immune systems, and whose origin is in progenitors of early ontogeny. We are trying to understand its role in the immune response through TLR receptors (“Toll-like receptors”) throughout life, particularly in animal models of bacterial infection, with the aim of designing new vaccine therapies. We analyze immune diversity in animal models and in human samples of children and elderly people diagnosed with respiratory disease, by: a) analyzing the lymphoid subpopulations of the local response. b) the study of the genetic rearrangements of the immunoglobulins that are produced. c) the systemic effector response and in peripheral organs. These approaches, carried out both in the mouse model and in human samples, will facilitate the design of more effective therapies and the characterization of biomarkers, adapted to the pediatric and elderly population, useful for the health field and technological innovation.rupo está interesado en los mecanismos de generación de células hematopoyéticas a lo largo de la ontogenia y la influencia que las primeras células hematopoyéticas, pertenecientes al sistema inmune innato/pseudoinnato, ejercen sobre el sistema inmune adaptativo en el individuo adulto. Nuestras líneas de investigación incluyen el análisis de poblaciones linfoides pseudoinnatas, que conectan el sistema inmune innato y adaptativo, y cuyo origen está en progenitores de la ontogenia temprana. Intentamos entender su papel en la respuesta inmune a través de receptores TLR (“Toll-like receptors”) a lo largo de la vida, en particular en modelos animales de infección bacteriana, con objeto de diseñar nuevas terapias vacunales. Analizamos la diversidad inmune en modelos animales y en muestras humanas de niños y ancianos diagnosticados de enfermedad respiratoria, mediante: a) el análisis de las subpoblaciones linfoides de la respuesta local. b) el estudio de los reordenamientos genéticos de las inmunoglobulinas que se producen. c) la respuesta efectora sistémica y en órganos periféricos. Estas aproximaciones, realizadas tanto en el modelo de ratón como en muestras humanas, facilitarán el diseño de terapias más eficaces y la caracterización de biomarcadores, adaptados a la población infantil y envejecida, de utilidad para el ámbito sanitario y de innovación tecnológica.

Doctoral theses

“Expression and functionality of tlr2 and tlr4 in lymphomyeloid populations present in the lung and lymphoid organs during embryonic and neonatal life in mouse models.”  Carolina Ruiz Sánchez. Complutense University of Madrid, 2022

Master's thesis

Detection and characterization of extracellular vesicles of platelet origin in lung supernatants from SPN-infected animals. Marta Paris, Alcalá University, 2024

Study of TLR-dependent activation in the RAW 264.7 macrophage line. Iñigo Merino de Saracho, Alcalá University, 2023

Study of the functionality of TLR receptors in the lung and other lymphoid organs in B cell populations using the neonatal mouse model. Yolanda Campanero, Alcalá University, 2023

Exploratory study of T lymphoid progenitors in the neonatal mouse lung. Alejandro Arrabal, Complutense University of Madrid, 2022

Study of B lymphoid differentiation in mice deficient for CD5 and CD6 molecules. Cristina Martín, Alcalá University, 2022

Role of platelets and their progenitor cells in two animal models of infection: SPN and RSV. Ana de Lucas Rius, Alcalá University, 2020

Pilot study of RSV infection in the mouse model: cellular phenotype of myeloid and lymphoid populations in the lung in two animal models of infection: SPN and RSV. Juan Antonio Martín Quesada, Alcalá University, 2020

B cell response during Streptococcus pneumoniae infection. Eva Castro, 2020

Study of the hematopoietic potential of neonatal lung cells in the mouse model. Ana Cogollo García, Alcalá University, 2018

Innate immune response to S. pneumoniae in the lung. Rodrigo Sánchez, Complutense University of Madrid, 2018

Neonatal immunity in the mouse model: localization and function of the innate and adaptive response. Alba Ezequiel Fernández, Alcalá University, 2017

Characterization of immunoglobulin gene diversity in the mouse model of TLR4 homeostasis and activation by bacterial lipopolysaccharide. Cristina García Caballero, Alcalá University, 2017

Altered lymphopoiesis and splenic B cell subsets on Telomerase Activity Deficient Mice (TERC-/-). Juliana Manosalva, Complutense University of Madrid, 2017

Study of the Immune Response in Nasopharyngeal Washings of Infants with Bronchiolitis. Isabel Martín Barrios, Complutense University of Madrid, 2016

Dynamics of B1-REL lymphocytes in the in vivo immunization model with DNP-LPS. Inmaculada Sanz Ramos University Alcalá, 2015

Megakaryocyte differentiation pathways in the mouse model. Marta Cobos Briz, Alcalá University, 2015

Role of megakaryocytes in infectious processes. Melania Guerrero Hue, Complutense University of Madrid, 2015

 

Final degree projects

Study of new bacterial vaccines in the mouse (Mus musculus) infection model due to Streptococcus pneumoniae. Alejandro Arrabal, Polytechnic University of Madrid, 2021

Megakaryocytes and platelets in SPN respiratory infection: Role of TLR4. Óscar González Hervás, Complutense University of Madrid, 2021

Study of the immune response mediated by pseudo-innate B lymphocytes against TLR4-dependent immunization models. Rodrigo Sánchez, Complutense University of Madrid, 2017.

Study of the diversity in the immunoglobulin repertoire in healthy individuals. Isabel Martín, Francisco de Vitoria University, 2015.

Dynamics of hematopoietic populations in the perinatal spleen. Inmaculada Sanz, Alcalá University, 2014

Teaching in training courses

Training course: Introduction to Flow Cytometry (from 2015 to present)
Training Course: Flow Cytometry Data Analysis (2018 to present)

Outreach / Citizen Science

• Collaboration in the 4th+Company CAM program.

• Collaboration with the ISCIII Scientific Culture Unit in Science Week at the ISCIII

• Scientific Dissemination Project "Talking about Science", carried out in Majadahonda primary, secondary and high school schools, since 2015 in collaboration with the Department of Education and Youth of the Majadahonda City Council: “How your Immune System works and healthy lifestyle habits to take care of it”

Doctoral theses

“Expression and functionality of tlr2 and tlr4 in lymphomyeloid populations present in the lung and lymphoid organs during embryonic and neonatal life in mouse models.”  Carolina Ruiz Sánchez. Complutense University of Madrid, 2022

Master's thesis

Detection and characterization of extracellular vesicles of platelet origin in lung supernatants from SPN-infected animals. Marta Paris, Alcalá University, 2024

Study of TLR-dependent activation in the RAW 264.7 macrophage line. Iñigo Merino de Saracho, Alcalá University, 2023

Study of the functionality of TLR receptors in the lung and other lymphoid organs in B cell populations using the neonatal mouse model. Yolanda Campanero, Alcalá University, 2023

Exploratory study of T lymphoid progenitors in the neonatal mouse lung. Alejandro Arrabal, Complutense University of Madrid, 2022

Study of B lymphoid differentiation in mice deficient for CD5 and CD6 molecules. Cristina Martín, Alcalá University, 2022

Role of platelets and their progenitor cells in two animal models of infection: SPN and RSV. Ana de Lucas Rius, Alcalá University, 2020

Pilot study of RSV infection in the mouse model: cellular phenotype of myeloid and lymphoid populations in the lung in two animal models of infection: SPN and RSV. Juan Antonio Martín Quesada, Alcalá University, 2020

B cell response during Streptococcus pneumoniae infection. Eva Castro, 2020

Study of the hematopoietic potential of neonatal lung cells in the mouse model. Ana Cogollo García, Alcalá University, 2018

Innate immune response to S. pneumoniae in the lung. Rodrigo Sánchez, Complutense University of Madrid, 2018

Neonatal immunity in the mouse model: localization and function of the innate and adaptive response. Alba Ezequiel Fernández, Alcalá University, 2017

Characterization of immunoglobulin gene diversity in the mouse model of TLR4 homeostasis and activation by bacterial lipopolysaccharide. Cristina García Caballero, Alcalá University, 2017

Altered lymphopoiesis and splenic B cell subsets on Telomerase Activity Deficient Mice (TERC-/-). Juliana Manosalva, Complutense University of Madrid, 2017

Study of the Immune Response in Nasopharyngeal Washings of Infants with Bronchiolitis. Isabel Martín Barrios, Complutense University of Madrid, 2016

Dynamics of B1-REL lymphocytes in the in vivo immunization model with DNP-LPS. Inmaculada Sanz Ramos University Alcalá, 2015

Megakaryocyte differentiation pathways in the mouse model. Marta Cobos Briz, Alcalá University, 2015

Role of megakaryocytes in infectious processes. Melania Guerrero Hue, Complutense University of Madrid, 2015

 

Final degree projects

Study of new bacterial vaccines in the mouse (Mus musculus) infection model due to Streptococcus pneumoniae. Alejandro Arrabal, Polytechnic University of Madrid, 2021

Megakaryocytes and platelets in SPN respiratory infection: Role of TLR4. Óscar González Hervás, Complutense University of Madrid, 2021

Study of the immune response mediated by pseudo-innate B lymphocytes against TLR4-dependent immunization models. Rodrigo Sánchez, Complutense University of Madrid, 2017.

Study of the diversity in the immunoglobulin repertoire in healthy individuals. Isabel Martín, Francisco de Vitoria University, 2015.

Dynamics of hematopoietic populations in the perinatal spleen. Inmaculada Sanz, Alcalá University, 2014

Teaching in training courses

Training course: Introduction to Flow Cytometry (from 2015 to present)
Training Course: Flow Cytometry Data Analysis (2018 to present)

Outreach / Citizen Science

• Collaboration in the 4th+Company CAM program.

• Collaboration with the ISCIII Scientific Culture Unit in Science Week at the ISCIII

• Scientific Dissemination Project "Talking about Science", carried out in Majadahonda primary, secondary and high school schools, since 2015 in collaboration with the Department of Education and Youth of the Majadahonda City Council: “How your Immune System works and healthy lifestyle habits to take care of it”

Content with Investigacion Infecciones Bacterianas Transmitidas por Agua y Alimentos .