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Investigation
Respiratory viruses and Influenza
Research Lines
Content with Investigacion .
Viral Biology
1. Study of the membrane fusion mechanism induced by the surface proteins of human respiratory syncytial virus and human metapneumovirus.
2. Study and characterization of the immune response to respiratory viruses.
3. Development of vaccines against human pneumoviruses based on protein subunits of the membrane fusion protein, protein F.
Publications
Lorente, E., E. Barnea, C. Mir, A. Admon, and D. López. 2020. The HLA-DP peptide repertoire from human respiratory syncytial virus is focused on major structural proteins with the exception of the viral polymerase. J Proteomics. 221:103759.
Lorente, E., E. Barnea, C. Mir, A. Admon, and D. López. 2020. The HLA-DP peptide repertoire from human respiratory syncytial virus is focused on major structural proteins with the exception of the viral polymerase. J Proteomics. 221:103759.
PUBMED DOIMarquez, A., M. Gomez-Fontela, S. Lauzurica, R. Candorcio-Simon, D. Munoz-Martín, M. Morales, M. Ubago, C. Toledo, P. Lauzurica, and C. Molpeceres. 2020. Fluorescence enhanced BA-LIFT for single cell detection and isolation. Biofabrication. 12:025019.
Marquez, A., M. Gomez-Fontela, S. Lauzurica, R. Candorcio-Simon, D. Munoz-Martín, M. Morales, M. Ubago, C. Toledo, P. Lauzurica, and C. Molpeceres. 2020. Fluorescence enhanced BA-LIFT for single cell detection and isolation. Biofabrication. 12:025019.
PUBMED DOILorente, E., M. Marcilla, P. G. de la Sota, A. Quijada-Freire, C. Mir, and D. López. 2021. Acid Stripping after Infection Improves the Detection of Viral HLA Class I Natural Ligands Identified by Mass Spectrometry. Int.J.Mol.Sci. 22.
Lorente, E., M. Marcilla, P. G. de la Sota, A. Quijada-Freire, C. Mir, and D. López. 2021. Acid Stripping after Infection Improves the Detection of Viral HLA Class I Natural Ligands Identified by Mass Spectrometry. Int.J.Mol.Sci. 22.
PUBMED DOIde la Sota, P. G., E. Lorente, L. Notario, C. Mir, O. Zaragoza, and D. López. 2021. Mitoxantrone Shows In Vitro, but Not In Vivo Antiviral Activity against Human Respiratory Syncytial Virus. Biomedicines. 9.
de la Sota, P. G., E. Lorente, L. Notario, C. Mir, O. Zaragoza, and D. López. 2021. Mitoxantrone Shows In Vitro, but Not In Vivo Antiviral Activity against Human Respiratory Syncytial Virus. Biomedicines. 9.
PUBMED DOIPredicted Epitope Abundance Supports Vaccine-Induced Cytotoxic Protection Against SARS-CoV-2 Variants of Concern.
Martín-Galiano, A. J., F. Diez-Fuertes, M. J. McConnell, and D. López. 2021. Predicted Epitope Abundance Supports Vaccine-Induced Cytotoxic Protection Against SARS-CoV-2 Variants of Concern. Front Immunol. 12:732693.
PUBMED DOIPrediction of Conserved HLA Class I and Class II Epitopes from SARS-CoV-2 Licensed Vaccines Supports T-Cell Cross-Protection against SARS-CoV-1.
López, D. 2022. Prediction of Conserved HLA Class I and Class II Epitopes from SARS-CoV-2 Licensed Vaccines Supports T-Cell Cross-Protection against SARS-CoV-1. Biomedicines. 10.
PUBMED DOIAbundance, Betweenness Centrality, Hydrophobicity, and Isoelectric Points Are Relevant Factors in the Processing of Parental Proteins of the HLA Class II Ligandome.
Lorente, E., A. J. Martín-Galiano, D. M. Kadosh, A. Barriga, J. Garcia-Arriaza, C. Mir, M. Esteban, A. Admon, and D. López. 2022. Abundance, Betweenness Centrality, Hydrophobicity, and Isoelectric Points Are Relevant Factors in the Processing of Parental Proteins of the HLA Class II Ligandome. J.Proteome.Res. 21:164-171.
DOICross-Recognition of SARS-CoV-2 B-Cell Epitopes with Other Betacoronavirus Nucleoproteins
Tajuelo, A., M. López-Siles, V. Mas, P. Perez-Romero, J. M. Aguado, V. Briz, M. J. McConnell, A. J. Martín-Galiano, and D. López. 2022. Cross-Recognition of SARS-CoV-2 B-Cell Epitopes with Other Betacoronavirus Nucleoproteins. Int.J.Mol.Sci. 23.
PUBMEDPredicted HLA Class I and Class II Epitopes From Licensed Vaccines Are Largely Conserved in New SARS-CoV-2 Omicron Variant of Concern.
López, D. 2022. Predicted HLA Class I and Class II Epitopes From Licensed Vaccines Are Largely Conserved in New SARS-CoV-2 Omicron Variant of Concern. Front Immunol. 13:832889.
PUBMEDGarcia-Arriaza, J., M. Esteban, and D. López. 2021
Garcia-Arriaza, J., M. Esteban, and D. López. 2021. Modified Vaccinia Virus Ankara as a Viral Vector for Vaccine Candidates against Chikungunya Virus. Biomedicines. 9.
PUBMEDDiagnósitico microbiológico y control de la legionelosis
Pelaz Antolín, C., et al., En Procedimientos en Microbiología Clínica, E.C.y.R. Cantón, Editor. 2005, SEIMC. p. 1-72.
PUBMEDLegionella-Biofilms-Amebas, un problema industrial, de sanidad ambiental y de salud pública
Juana María González-Rubio, Celia Játiva, Almudena Cascajero, Fernando González-Camacho. Infoplagas, nº 112, agosto 2023 pags: 20-24. (Artículo de divulgación).
DOIPrograma de Legionelosis. En Echevarría Mayo JE y Oteo Iglesias J (Editores). Programas de Vigilancia Microbiológica pags: 74-80. Centro Nacional de Microbiología, Madrid: Instituto de Salud Carlos III, 2021.
Bellido B y Pelaez C: Programa de Legionelosis. En Echevarría Mayo JE y Oteo Iglesias J (Editores). Programas de Vigilancia Microbiológica pags: 74-80. Centro Nacional de Microbiología, Madrid: Instituto de Salud Carlos III, 2021.
Programa de Legionelosis. En Echevarría Mayo JE y Oteo Iglesias J (Editores) Programas de Vigilancia Microbiológica Centro Nacional de Microbiología.
Fernando González-Camacho y Almudena Cascajero. Programa de Legionelosis. En Echevarría Mayo JE y Oteo Iglesias J (Editores) Programas de Vigilancia Microbiológica Centro Nacional de Microbiología. Volumen 2:77-89. 2021-2022 Majadahonda (Madrid); Instituto de Salud Carlos III, Centro Nacional de Microbiología: 2023.
Chikungunya virus infections among travellers returning to Spain, 2008 to 2014
3. Maria Dolores Fernandez Garcia; Mathieu Bangert; Fernando de Ory; Arantxa Potente; Lourdes Hernandez; Fatima Lasala; Laura Herrero; Francisca Molero; Anabel Negredo; Ana Vázquez; Teodora Minguito; Pilar Balfagón; Jesus de la Fuente; Sabino Puente; Eva Ramírez de Arellano; Mar Lago; Miguel Martinez; Joaquim Gascón; Francesca Norman; Rogelio Lopez Velez; Elena Sulleiro; Diana Pou; Nuria Serre; Ricardo Fernández Roblas; Antonio Tenorio; Leticia Franco; Maria Paz Sanchez Seco. Chikungunya virus infections among travellers returning to Spain, 2008 to 2014. Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin. 21 - 36, (Sweden): 08/09/2016. ISSN 1560-7917
PUBMED DOILegionella feeleii: Ubiquitous Pathogen in the Environment and Causative Agent of Pneumonia
Vaccaro L, Gomes TS, Izquierdo F, Magnet A, Llorens Berzosa S, Ollero D, Salso S, Alhambra A, Gómez C, López Cano M, Pelaz C, Bellido Samaniego B, Del Aguila C, Fenoy S, Hurtado-Marcos C. Front Microbiol. 2021;12:707187.
DOIAdditional Information
The Respiratory Viruses and Influenza Unit is the oldest WHO Influenza Center in our country (1968), designated as the National Influenza Center by the Ministry of Health in 1971, and is the national coordinator of the CCAA laboratories in the National Influenza Surveillance Network of the Influenza Surveillance System in Spain.
Its general objective is virological surveillance, detection and control of influenza in Spain. Since 1992, together with the Pediatric Service of the Severo Ochoa Hospital, a line of research has been developed based on the etiological study of viral respiratory infections in hospitalized children.
Since 2015, the La Paz Children's University Hospital and the Immunology Laboratory of the IIS-Fundación Jiménez Díaz have also participated, expanding the study to newborns and neonatal units.
To comprehensively study respiratory viruses and the response of the immune system in viral respiratory infections that affect newborns and pediatric patients.
Deepen the knowledge of the biological properties of the viruses involved in the disease: dependency/facilitation, balance between viruses and their adaptation in multiple infections, relationship with severity, existence of virome imbalance.
The Respiratory Viruses and Influenza Unit is the oldest WHO Influenza Center in our country (1968), designated as the National Influenza Center by the Ministry of Health in 1971, and is the national coordinator of the CCAA laboratories in the National Influenza Surveillance Network of the Influenza Surveillance System in Spain.
Its general objective is virological surveillance, detection and control of influenza in Spain. Since 1992, together with the Pediatric Service of the Severo Ochoa Hospital, a line of research has been developed based on the etiological study of viral respiratory infections in hospitalized children.
Since 2015, the La Paz Children's University Hospital and the Immunology Laboratory of the IIS-Fundación Jiménez Díaz have also participated, expanding the study to newborns and neonatal units.
To comprehensively study respiratory viruses and the response of the immune system in viral respiratory infections that affect newborns and pediatric patients.
Deepen the knowledge of the biological properties of the viruses involved in the disease: dependency/facilitation, balance between viruses and their adaptation in multiple infections, relationship with severity, existence of virome imbalance.
The current director of CNM is Dr. José Miguel Rubio Muñoz.
Dr. José Miguel Rubio has a degree in Biological Sciences from the Universidad Autónoma de Madrid (1986) and a PhD in Biological Sciences from the same university (1992). He carried out his doctoral thesis at the Department of Genetics of the Universidad Autónoma de Madrid, as Associate Professor (1988-1989), and at the School of Biology of the University of East Anglia in Norwich, UK, as Senior Research Assistant (1989-1992).
During his postdoctoral period he obtained a grant from the European Commission within the Human Capital and Mobility Program to be carried out at the University of “La Sapienza” in Rome, Italy and the Institute of Molecular Biology and Biotechnology in Crete, Greece (1993-1994). Subsequently, he made a further stay funded by the WHO and the university itself at the Department of Entomology, Wageningen University, The Netherlands (1994-1996).
Since 1997 he has been a member of the Instituto de Salud Carlos III (ISCIII), where he joined the Department of Parasitology of the National Center of Microbiology, as an EU-INCO postdoctoral fellow and later with a grant from the Autonomous Community of Madrid (CAM). She was part of the founding group of the National Center for Tropical Medicine (2003-2006) and of the 24/7 Alerts and Emergencies Unit (2006-2018) and is currently Head of the Malaria and Emerging Parasitosis Unit of the National Microbiology Center and is part, as research staff, of the Center for Biomedical Research Network on Infectious Diseases (CIBERINFEC/ISCIII).
During his scientific career he has been Visiting Scientist at the Leonidas e Marie Dean Center (FIOCRUZ-AMAZONAS, Manaus, Brazil) and is an External Consultant of the Parasitology Departments of Cairo University (Egypt) and the Medical Research Center (MRC) of Kuala Lumpur (Malaysia). He also belongs or has belonged to different national and international committees: Member of the expert group for malaria control of the European Centre for Disease Control (ECDC) since 2011; Expert-Evaluator for health programs of the European Commission since 2004; Spanish Representative (commissioned by ISCIII and MSC) in the Technical Scientific Committee of the TDR (WHO) 2007-2008; Spanish Deputy Focal Point for microbiology at the European Centre for Disease Control (ECDC) from 2012 to 2020; and, member of the Research Ethics Committee of ISCIII until 2019.
In this period he has published more than 100 articles in international indexed journals, 10 book chapters and has been co-editor of two books in the area of malaria, tropical medicine and neglected diseases. He has participated in 58 competitively funded research projects, 20 of them international, having been the principal investigator in 8 national and 11 international projects as PI of the project or WP leader. In addition, he has led five agreements with companies. Currently he has been awarded four sexenios of research, being presented this year 2025 to the fifth. In the teaching field, he participates in different postgraduate programs in the areas of microbiology and parasitology, having directed seven doctoral theses and more than 20 Master's or Degree final projects, both nationally and internationally.
El laboratorio de Referencia e Investigación en Resistencia a Antibióticos ofrece una amplia cartera de servicios al Sistema Nacional de Salud, las cuales pueden solicitarse en cnm-laboratorios.isciii.es. Jefe del Laboratorio: Jesús Oteo Iglesias (Punto focal Nacional de Resistencia antibiótica).
Dispone de dos programas de Vigilancia oficiales y gratuitos que engloban los ensayos ofertados ya sea como aislamientos individuales o mediante estudio de brotes. El Laboratorio utiliza asimismo técnicas de PCR en tiempo real para la detección de genes de resistencia, estas técnicas se han adaptado a un formato multiplex que permite detectar varios genes en la misma reacción. En los últimos años se han incluido metodologías basadas en la secuenciación de genomas completos para el análisis de bacterias multiresistentes (WGS).
Programa de vigilancia de Haemophilus influenzae. Responsables: María Pérez Vázquez (Punto focal Nacional de Haemophilus influenzae) y Belén Aracil. Laboratorio encargado de la identificación, estudio de sensibilidad y análisis genotípico de aislados de Haemophilus influenzae, centrándose esencialmente en la patología invasiva debida este patógeno.
Programa de vigilancia de Resistencia a Antibióticos. Responsables: María Pérez Vázquez y Belén Aracil (Punto focal Nacional de Resistencia antibiótica). Laboratorio encargado de la identificación, el estudio de sensibilidad antibiótica, y el diagnóstico fenotípico y genotípico de los diferentes mecanismos de resistencia a antibióticos fundamentalmente en enterobacterias y gram-negativos no fermentadores y Enterococcus spp.
Estudio de brotes. Responsables: Belén Aracil y María Pérez Vázquez. El programa incluye la caracterización de brotes nosocomiales y clones emergentes de alto riesgo mediante diferentes técnicas moleculares (tabla resumen). Éstas, nos permiten realizar estudios filogenéticos con el fin de obtener una información detallada acerca la relación entre los diferentes aislados y su trazabilidad. El objetivo final es generar datos que se transfieren a los hospitales como ayuda para la prevención o control de la propagación del brote.
Acreditación y Calidad. Responsable: Belén Aracil. El laboratorio Referencia e Investigación en Resistencia a Antibióticos ha sido de los primeros en el ISCIII en la utilización de técnicas acreditadas por la Entidad Nacional de Acreditaciones (ENAC). Este laboratorio consiguió la primera acreditación homologada de técnicas diagnósticas en 2012, programa que ha sido ampliado, de manera que en la actualidad más de la mitad de las técnicas ofrecidas al Sistema Nacional de Salud están debidamente acreditadas por ENAC.
Técnicos responsables de las técnicas realizadas en el Laboratorio: Noelia Lara Fuella y Verónica Bautista Sánchez.
En la siguiente imagen se resumen las técnicas ofrecidas al Sistema Nacional de Salud.
| PROGRAMAS | NOMBRE CARTERA SERVICIO | PATÓGENO | DETERMINACIÓN, DETECCIÓN, ANÁLISIS | MÉTODOS |
|
Programa de vigilancia de Haemophilus Programa de vigilancia de resistencia a antibióticos. |
Identificación bacteriana |
Haemophilus sp. Enterobacterias, gram-negativos no fermentadores, Enterococcus spp |
Identificación bacteriana |
Bioquímicos MALDI TOF Secuenciación de RNAr |
| | Identificación capsular |
Haemophilus influenzae
|
Identificación capsular fenotípica y genotípica |
Aglutinación serológica en latex PCR ind/multiplex |
| | Determinación de Sensibilidad |
Haemophilus sp. Enterobacterias, gram-negativos no fermentadores, Enterococcus
|
Determinación de Sensibilidad |
Microdilución Tiras epsilon Kirby Bauer |
| | Métodos fenotípicos de detección de mecanismos de resistencia |
Enterobacterias, gram-negativos no fermentadores,
|
Métodos fenotípicos de detección de mecanismos de resistencia |
Discos y tabletas combinados con inhibidores Tiras combinadas Test de Hodge modificado CabaNP Inmunocromatografía CBP |
| | Métodos genotípicos de detección de mecanismos de resistencia |
Haemophilus sp. Enterobacterias, gram-negativos no fermentadores, Enterococcus
|
ADN, PCR y secuenciación |
PCR ind/multiplex Análisis comparativo de las secuencias |
| | Tipificación molecular/análisis filogenéticos |
Haemophilus sp. Enterobacterias, gram-negativos no fermentadores, Enterococcus
|
Corte enzimas de restricción, electroforesis ADN, PCR y secuenciación Preparación de librerías y secuenciación y análisis de genomas completos |
PFGE
MLST
WGS |