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Investigación

Toxoplasmosis and intestinal protozoa

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Toxoplasmosis y Protozoos intestinales

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Performance of Panfungal- and Specific-PCR-Based Procedures for Etiological Diagnosis of Invasive Fungal Diseases on Tissue Biopsy Specimens with Proven Infection: a 7-Year Retrospective Analysis from a Reference Laboratory

9. Buitrago MJ, Bernal-Martinez L, Castelli MV, Rodriguez-Tudela JL, Cuenca-Estrella M Performance of panfungal--and specific-PCR-based procedures for etiological diagnosis of invasive fungal diseases on tissue biopsy specimens with proven infection: a 7-year retrospective analysis from a reference laboratory. J Clin Microbiol. 2014 May;52(5):1737-40. doi: 10.1128/JCM.00328-14. Epub 2014 Feb 26.PMID: 24574295.

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Epidemiología actual y diagnóstico de laboratorio de las micosis endémicas en España

11. Buitrago MJ, Cuenca-Estrella M. [Current epidemiology and laboratory diagnosis of endemic mycoses in Spain]. Enferm Infecc Microbiol Clin. 2012 Aug;30(7):407-13. doi: 10.1016/j.eimc.2011.09.014. Epub 2011 Nov 29. PMID: 22130575 Review. Spanish.

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A matrix-assisted laser desorption/ionization time of flight mass spectrometry reference database for the identification of Histoplasma capsulatum

12. Buitrago MJ, Bernal-Martínez L, Castelli MV, Rodríguez-Tudela JL, Cuenca-Estrella M. Histoplasmosis and paracoccidioidomycosis in a non-endemic area: a review of cases and diagnosis. J Travel Med. 2011 Jan-Feb;18(1):26-33. doi: 10.1111/j.1708-8305.2010.00477.x. Epub 2010 Nov 28. PMID: 21199139.

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Copy Number Variation of Mitochondrial DNA Genes in Pneumocystis jirovecii According to the Fungal Load in BAL Specimens

13. Valero C, Buitrago MJ, Gago S, Quiles-Melero I, García-Rodríguez J. A matrix-assisted laser desorption/ionization time of flight mass spectrometry reference database for the identification of Histoplasma capsulatum. Med Mycol. 2018 Apr 1;56 (3):307-314. doi: 10.1093/mmy/myx047. PMID: 28992262.

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Content with Investigacion Toxoplasmosis y Protozoos intestinales .

List of staff

Información adicional

Our group carries out research studies in the diagnosis, reference and epidemiology of zoonoses and emerging diseases, both indigenous and imported, caused by protozoa. Coordinates the study in the human field with the relevance of the animal field and the environment (One Health initiative), with special interest in Toxoplasmosis, a highly prevalent zoonosis (WHO lists it as the 3rd food-borne zoonosis in Europe), presents a complex epidemiological cycle and causes neurological, ocular and systemic symptoms. We carry out diagnostic and characterization studies of Toxoplasma gondii from human and animal cases, to obtain greater epidemiological information and analyze the possible relationship with virulence and pathology.

Cryptosporidium, Giardia, Blastocystis and Entamoeba histolytica cause gastrointestinal diseases, affecting children, immunosuppressed people and travelers. They can cause outbreaks.  We develop diagnostic and characterization studies of isolates from humans and animals, from different areas and countries, to establish the presence of the main species and genotypes and the epidemiological situation. We are beginning the study of associations between these parasites and the intestinal microbiota.

The pathogenic Free-Living Amoebas, Acanthamoeba, Naegleria fowleri and Balamuthia mandrillaris, cause emerging diseases, highlighting the importance of the environment in transmission. They cause underdiagnosed neurological and ocular cases. The diagnostic and genotyping study of human and animal isolates that we are carrying out aims to establish the real prevalence, transmission routes and epidemiology.

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