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Investigation
Bacterial Taxonomy
Research Lines
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Hepatitis
- Diseño de métodos diagnósticos para el estudio de los virus de las hepatitis (VH) A, B, C, D, E: Diseñamos sistemas de PCR para su detección y caracterización.
- Evaluación de métodos diagnósticos de los VH. Colaboramos con empresas para estudios de sensibilidad y especificidad de equipos diagnósticos.
- Estudios de Seroprevalencia de los virus de las hepatitis.
- Epidemiología genómica de genomas completos de VHA, VHB, VHC, VHD y VHE en colaboración con el ECDC. Estudios de trazabilidad del VHE.
- Caracterización molecular de virus de las hepatitis mediante secuenciación masiva: a) VHB: mutantes de escape HBsAg (prevalencia y efectos en la detección del HBsAg). Estudio de mutaciones en epítopos de estimulación inmune y mutaciones asociadas a evolución clínica desfavorable.
- b) VHC: resistencias a los antivirales de acción directa. Análisis molecular de subtipos poco frecuentes.
c) Estudios filogenéticos del VHD.
d) Análisis genómico del VHE.
e) Investigación etiológica de hepatitis no filiadas mediante estudios de metagenómica.
- b) VHC: resistencias a los antivirales de acción directa. Análisis molecular de subtipos poco frecuentes.
Research projects
Content with Investigacion .
1. Proyecto CIBEREPS 2022. Microbiological and genomic investigation of hepatitis in children by metagenomic approach in case and control subjects (IP: Ana Avellón).
2023-2024. En colaboración con el Hospital San Joan de Deu de Barcelona.
2. MPY 501-19: Tracking hepatitis E virus infection by means of epidemiological research and whole genome sequencing. Project TrazHE. (IP: Ana Avellón). 2020-2024.
3. Proyecto CIBEREPS 2021 Metagenomic sequencing to identify viral aetiologies in undiagnosed paediatric cases of meningitis and encephalitis (IP: D. Tarragó). 2021-2022.
4. MPY 383/19 (PEJ2018-004446-A). Ayudas para la promoción de empleo joven e implantación de la garantía juvenil en I+D+I. análisis de la complejidad de secuencias de los virus de la hepatitis A, B, C; D y E (VHA, VHB, VHC, VHD y VHE) mediante técnicas de secuenciación masiva. (IP: Ana Avellón). 2020-2021.
5. MPY 1285/16 Movilidad "Salvador de Madariaga" programa estatal de promoción de talento y su empleabilidad. (IP: Ana Avellón). 2016.
Publications
Prevalence and genotype identification of Toxoplasma gondii in wild animals from southwestern Spain.
8. Calero-Bernal R, Saugar JM, Frontera E, Pérez-Martín JE, Habela MA, Serrano FJ, Reina D, Fuentes I. 2015. Prevalence and genotype identification of Toxoplasma gondii in wild animals from southwestern Spain. J Wildl Dis, 51:233-8.
PUBMED DOIHigh SARS-CoV-2 Viral Load and Low CCL5 Expression Levels in the Upper Respiratory Tract Are Associated With COVID-19 Severity.
4. Pérez-García F, Martin-Vicente M, Rojas-García RL, Castilla-García L, Muñoz-Gomez MJ, Hervás-Fernández I, González-Ventosa V, Vidal-Alcántara EJ, Cuadros-González J, Bermejo-Martin JF (‡), Resino S (‡ *), Martínez I (‡). High SARS-CoV-2 Viral Load and Low CCL5 Expression Levels in the Upper Respiratory Tract Are Associated With COVID-19 Severity. J Infect Dis 2022; 225(6):977-982 (A; FI= 7.76; Q1, Infectious Diseases; JCR 2021). PMID: 34910814 DOI: 10.1093/infdis/jiab604.
PUBMED DOIMetabolomic changes after DAAs therapy are related to the improvement of cirrhosis and inflammation in HIV/HCV-coinfected patients.
5. Virseda-Berdices A, Rojo D, Martínez I, Berenguer J, González-García J, Brochado-Kith O, Fernández-Rodríguez A, Díez C, Hontañon V, Pérez-Latorre L, Micán R, Barbas C, Resino S (‡ *), Jiménez-Sousa MA (‡ *). Metabolomic changes after DAAs therapy are related to the improvement of cirrhosis and inflammation in HIV/HCV-coinfected patients. Biomed Pharmacother 2022, 147: 112626. (A; FI= 7.42; D1, Pharmacology & Pharmacy; JCR 2021).
PUBMED DOIBlood microbiome is associated with changes in portal hypertension after successful direct-acting antiviral therapy in patients with HCV-related cirrhosis.
7. Virseda-Berdices A, Brochado-Kith O, Díez C, Hontañon V, Berenguer J, González-García J, Rojo D, Fernández-Rodríguez A, Ibañez-Samaniego L, Llop-Herrera E, Olveira A, Perez-Latorre L, Barbas C, Rava M (‡), Resino S (‡ *), Jiménez-Sousa MA (‡ *). Blood microbiome is associated with changes in portal hypertension after successful direct-acting antiviral therapy in patients with HCV-related cirrhosis. J Antimicrob Chemoth 2022; 77 (3): 719–726 (A; FI= 5.76; Q1, Pharmacology & Pharmacy; JCR 2020).
PUBMED DOIA Q Fever Outbreak with a High Rate of Abortions at a Dairy Goat Farm: Coxiella burnetii Shedding, Environmental Contamination, and Viability
3. Álvarez-Alonso R, Basterretxea M, Barandika JF, Hurtado A, Idiazabal J, Jado I, Beraza X, Montes M, Liendo P, García-Pérez AL. A Q Fever Outbreak with a High Rate of Abortions at a Dairy Goat Farm: Coxiella burnetii Shedding, Environmental Contamination, and Viability. Appl Environ Microbiol. 2018 Oct 1;84(20).
PUBMED DOIIrruptive mammal host populations shape tularemia epidemiology.
4. Luque-Larena, Juan J.; Mougeot, Francois; Arroyo, Beatriz; Dolors Vidal, Ma; Rodriguez-Pastor, Ruth; Escudero, Raquel; Anda, Pedro; Lambin, Xavier. Irruptive mammal host populations shape tularemia epidemiology. Plos Pathogens. 13 - 11, Public Library Science, 01/11/2017.
PUBMED DOIEnvironmental sampling coupled with real-time PCR and genotyping to investigate the source of a Q fever outbreak in a work setting.
5. Hurtado A, Alonso E, Aspiritxaga I, López Etxaniz I, Ocabo B, Barandika JF, Fernández-Ortiz DE Murúa JI, Urbaneja F, Álvarez-Alonso R, Jado I, García-Pérez AL. Environmental sampling coupled with real-time PCR and genotyping to investigate the source of a Q fever outbreak in a work setting. Epidemiol Infect. 2017 Jul;145(9):1834-1842.
PUBMED DOIDensity-Dependent Prevalence of Francisella tularensis in Fluctuating Vole Populations, Northwestern Spain
6. Rodriguez-Pastor, Ruth; Escudero, Raquel; Vidal, Dolors; Mougeot, Francois; Arroyo, Beatriz; Lambin, Xavier; Maria Vila-Coro, Ave; Rodriguez-Moreno, Isabel; Anda, Pedro; Luque-Larena, Juan J.Density-Dependent Prevalence of Francisella tularensis in Fluctuating Vole Populations, Northwestern Spain. Emerging Infectious Diseases. 23 - 8, pp. 1377 - 1379. Centers Disease Control, 01/08/2017.
PUBMED DOIGenotypes of Coxiella burnetii in wildlife: disentangling the molecular epidemiology of a multi-host pathogen
7. González-Barrio D, Jado I, Fernández-de-Mera IG, Del Rocio Fernández-Santos M, Rodríguez-Vargas M, García-Amil C, Beltrán-Beck B, Anda P, Ruiz-Fons F. Genotypes of Coxiella burnetii in wildlife: disentangling the molecular epidemiology of a multi-host pathogen. Environ Microbiol Rep. 2016 Oct;8(5):708-714.
PUBMED DOIDevelopment of Improved Serodiagnostics for Tularemia by Use of Francisella tularensis Proteome Microarrays
8. Nakajima, Rie; Escudero, Raquel; Molina, Douglas M.; Rodriguez-Vargas, Manuela; Randall, Arlo; Jasinskas, Algis; Pablo, Jozelyn; Felgner, Philip L.; AuCoin, David P.; Anda, Pedro; Davies, D. Huw. Towards Development of Improved Serodiagnostics for Tularemia by Use of Francisella tularensis Proteome Microarrays. Journal of Clinical Microbiology. 2016 Jul;54(7):1755-1765.
PUBMED DOIInterruption of onchocerciasis transmission in Bioko Island: Accelerating the movement from control to elimination in Equatorial Guinea
5. Herrador Z, Garcia B, Ncogo P, Perteguer MJ, Rubio JM, Rivas E, Cimas M, Ordoñez G, de Pablos S, Hernández-González A, Nguema R, Moya L, Romay-Barja M, Garate T, Barbre K, Benito A. Interruption of onchocerciasis transmission in Bioko Island: Accelerating the movement from control to elimination in Equatorial Guinea. PLoS Negl Trop Dis. 2018 May 3;12(5):e0006471.
PUBMED DOILAMP kit for diagnosis of non-falciparum malaria in Plasmodium ovale infected patients
7. Thuy-Huong Ta-Tang, Sergio L. B. Luz, Francisco J. Merino, Isabel de Fuentes, Rogelio López-Vélez, Tatiana A. P. Almeida, Marta Lanza, Cláudia M. M. Abrahim, and José M. Rubio (2016). Atypical Mansonella ozzardi Microfilariae from an Endemic Area of Brazilian Amazonia. Am. J. Trop. Med. Hyg 95(3), 2016, pp. 633–636.
PUBMED DOIComparison of Imported Plasmodium ovale curtisi and P. ovale wallikeri Infections among Patients in Spain, 2005-2011.
9. Rojo-Marcos G, Rubio-Muñoz JM, Ramírez-Olivencia G, García-Bujalance S, Elcuaz-Romano R, Díaz-Menéndez M, Calderón M, García-Bermejo I, Ruiz-Giardín JM, Merino-Fernández FJ, Torrús-Tendero D, Delgado-Iribarren A, Ribell-Bachs M,Arévalo-Serrano J, Cuadros-González J (2014). Comparison of Imported Plasmodium ovale curtisi and P. ovale wallikeri Infections among Patients in Spain, 2005-2011. Emerg Infect Dis. 2014 Mar;20(3):409-16.
PUBMED DOIArbovirus surveillance: first dengue virus detection in local Aedes albopictus mosquitoes in Europe, Catalonia, Spain, 2015.
1. C Aranda; MJ Martínez; T Montalvo; R Eritja; J Navero-Castillejos; E Herreros; E Marqués; R Escosa; I Corbella; E Bigas; L Picart; M Jané; I Barrabeig; N Torner; S Talavera; Ana Vázquez; María Paz Sánchez-Seco; Nuria Busquets. Arbovirus surveillance: first dengue virus detection in local Aedes albopictus mosquitoes in Europe, Catalonia, Spain, 2015.Eurosurveillance. 23 - 47, 2018.
PUBMED DOIPhylogenetic Characterization of Crimean-Congo Hemorrhagic Fever Virus, Spain
2. Eva Ramírez de Arellano; Lourdes Hernández; M José Goyanes; Marta Arsuaga; Ana Fernández Cruz; Anabel Negredo; María Paz Sánchez Seco. Phylogenetic Characterization of Crimean-Congo Hemorrhagic Fever Virus, Spain. Emerging infectious diseases. 23 - 12, pp. 2078 - 2080. 12/2017. ISSN 1080-6059
PUBMED DOIToscana virus infection in Catalonia (Spain).
4. Neus Cardeñosa; Diana Kaptoul; Pedro Fernández Viladrich; Carles Aranda; Fernando de Ory; Jordi Niubó; Pere Plans; Angela Domínguez; Giovanni Fedele; Antonio Tenorio; María Paz Sánchez Seco. Toscana virus infection in Catalonia (Spain). Vector borne and zoonotic diseases (Larchmont, N.Y.). 13 - 4, pp. 273 - 278. 04/2013. ISSN 1557-7759
PUBMED DOI. Autochthonous Crimean-Congo Hemorrhagic Fever in Spain
5. Anabel Negredo; Fernando de la Calle Prieto; Eduardo Palencia Herrejón; Marta Mora Rillo; Jenaro Astray Mochales; María P Sánchez Seco; Esther Bermejo Lopez; Javier Menárguez; Ana Fernández Cruz; Beatriz Sánchez Artola; Elena Keough Delgado; Eva Ramírez de Arellano; Fátima Lasala; Jakob Milla; Jose L Fraile; Maria Ordobás Gavín; Amalia Martinez de la Gándara; Lorenzo López Perez; Domingo Diaz Diaz; M Aurora López García; Pilar Delgado Jimenez; Alejandro Martín Quirós; Elena Trigo; Juan C Figueira; Jesús Manzanares; Elena Rodriguez Baena; Luis Garcia Comas; Olaia Rodríguez Fraga; Nicolás García Arenzana; Maria V Fernández Díaz; Victor M Cornejo; Petra Emmerich; Jonas Schmidt Chanasit; Jose R Arribas. Autochthonous Crimean-Congo Hemorrhagic Fever in Spain.The New England journal of medicine. 377 - 2, pp. 154 - 161. 13/07/2017. ISSN 1533-4406
PUBMED DOIZika Virus Screening among Spanish Team Members After 2016 Rio de Janeiro, Brazil, Olympic Games
6. Natalia Rodriguez Valero; Alberto M Borobia; Mar Lago; Maria Paz Sánchez Seco; Fernando de Ory; Ana Vázquez; Jose Luis Pérez Arellano; Cristina Carranza Rodríguez; Miguel J Martínez; Alicia Capón; Elias Cañas; Joaquin Salas Coronas; Arkaitz Azcune Galparsoro; Jose Muñoz. Zika Virus Screening among Spanish Team Members After 2016 Rio de Janeiro, Brazil, Olympic Games. Emerging infectious diseases. 23 - 8, pp. 1426 - 1428. 08/2017. ISSN 1080-6059
PUBMED DOIProlonged Zika Virus Viremia during Pregnancy
7. Anna Suy; Elena Sulleiro; Carlota Rodó; Élida Vázquez; Cristina Bocanegra; Israel Molina; Juliana Esperalba; María P Sánchez Seco; Hector Boix; Tomás Pumarola; Elena Carreras. Prolonged Zika Virus Viremia during Pregnancy. The New England journal of medicine. 375 - 26, pp. 2611 - 2613. 29/12/2016. ISSN 1533-4406
PUBMED DOIAdditional Information
The research objectives focus on the areas of Bacterial Taxonomy and Microbiology in Public Health:
- Identification of pathogenic bacteria that are difficult to assign taxonomically
- Description of new species
- Study of bacterial population biology through typing
- Epidemiology of antimicrobial resistance and virulence factors for certain bacterial pathogens in different nosocomial/community settings.
These objectives are addressed from the perspective of polyphasic taxonomy (phenotypic and genotypic taxonomy) through the study of targets for genus/species assignment and phylogenetic analyses. The analysis of the virulome and resistome of certain species is approached from the perspective of complete genome sequencing.
Research on invasive infection by Streptococcus pyogenes and other beta-hemolytic streptococci has been carried out since 1994, through the Surveillance Program of the National Center for Microbiology. The typing of invasive S. pyogenes strains circulating in Spain includes determination of: serotype (emm-type); the genetic profile of streptococcal pyrogenic exotoxins; the antimicrobial resistance phenotype; and the genotypes of epidemiologically related strains and/or outbreaks.
The research objectives focus on the areas of Bacterial Taxonomy and Microbiology in Public Health:
- Identification of pathogenic bacteria that are difficult to assign taxonomically
- Description of new species
- Study of bacterial population biology through typing
- Epidemiology of antimicrobial resistance and virulence factors for certain bacterial pathogens in different nosocomial/community settings.
These objectives are addressed from the perspective of polyphasic taxonomy (phenotypic and genotypic taxonomy) through the study of targets for genus/species assignment and phylogenetic analyses. The analysis of the virulome and resistome of certain species is approached from the perspective of complete genome sequencing.
Research on invasive infection by Streptococcus pyogenes and other beta-hemolytic streptococci has been carried out since 1994, through the Surveillance Program of the National Center for Microbiology. The typing of invasive S. pyogenes strains circulating in Spain includes determination of: serotype (emm-type); the genetic profile of streptococcal pyrogenic exotoxins; the antimicrobial resistance phenotype; and the genotypes of epidemiologically related strains and/or outbreaks.