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Vaccination with LytA, LytC, or Pce of Streptococcus pneumoniae Protects against Sepsis by Inducing IgGs That Activate the Complement System

Corsini B, Aguinagalde L, Ruiz S, Domenech M, Yuste J. Vaccination with LytA, LytC, or Pce of Streptococcus pneumoniae Protects against Sepsis by Inducing IgGs That Activate the Complement System. Vaccines. 2021 Feb 23;9(2):186.

PUBMED DOI

Physiologic and transcriptomic effects triggered by overexpression of wild type and mutant DNA topoisomerase I in Streptococcus pneumoniae

García-López M, Hernández P, Megias D, Ferrándiz MJ, de la Campa AG. Int J Mol Sci. 2023; 24:15800.

PUBMED DOI

StaR Is a positive regulator of topoisomerase I activity involved in supercoiling maintenance in Streptococcus pneumoniae

de Vasconcelos Junior AA, Tirado-Vélez JM, Martín-Galiano AJ, Megias D, Ferrándiz MJ, Hernández P, Amblar M, de la Campa AG. Int J Mol Sci. 2023; 24:5973.

PUBMED DOI

Role of PatAB transporter in efflux of levofloxacin in Streptococcus pneumoniae

Amblar M, Zaballos A, de la Campa AG. Antibiotics. 2022; 17:1837.

PUBMED DOI

Seconeolitsine, the novel inhibitor of DNA topoisomerase I, protects against invasive pneumococcal disease caused by fluoroquinolone-resistant strains.

Tirado-Vélez JM, Carreño D, Sevillano D, Alou L, Yuste J, de la Campa AG. Antibiotics 2021; 10:573.

PUBMED DOI

Genome-wide proximity between RNA polymerase and DNA topoisomerase I supports transcription in Streptococcus pneumoniae

Ferrándiz M-J, Hernández P, de la Campa AG. PLoS Genet. 2021; 17:e1009542.

PUBMED DOI

A Small Non-Coding RNA Modulates Expression of Pilus-1 Type in Streptococcus pneumoniae

Acebo P, Herranz C, Bernal-Espenberger L, Gómez-Sanz A, Terron MC, Luque D and Amblar M. Microorganisms. 2021; 9:1883.

PUBMED DOI

Reactive oxygen species production is a major factor directing the post-antibiotic effect of fluoroquinolones in Streptococcus pneumoniae

García MT, Valenzuela MV, Ferrándiz MJ, de la Campa AG. Antimicrob Agents Chemother. 2019; 63:e00737-19.

PUBMED DOI

HU of Streptococcus pneumoniae is essential for the preservation of DNA supercoiling

Ferrándiz MJ, Carreño D, Ayora S, de la Campa AG. Front Microbiol. 9:493 (2018).

PUBMED DOI

Boldine-derived alkaloids inhibit the activity of DNA topoisomerase I and growth of Mycobacterium tuberculosis.

García MT, Carreño D, Tirado-Vélez JM, Ferrándiz MJ, Rodrigues L, Gracia B, Amblar M, Ainsa JA*, de la Campa AG. Front Microbiol. 9:493 (2018).

PUBMED DOI

Absence of tmRNA has a protective effect against fluoroquinolones in Streptococcus pneumoniae

Brito L, Wilton J, Ferrándiz MJ, Gómez-Sanz A, de la Campa AG, Amblar M. Front. Microbiol. 7:2164 (2017).

PUBMED DOI

Bridging chromosomal architecture and pathophysiology of Streptococcus pneumoniae

Martín-Galiano AJ, Ferrándiz MJ, de la Campa AG. Genome Biol Evol. 2017; 9:350-361.

PUBMED DOI

Upregulation of the PatAB transporter confers fluoroquinolone resistance to Streptococcus pseudopneumoniae

Alvarado M, Martín-Galiano AJ, Ferrándiz MJ, Zaballos A, de la Campa AG. Front Microbiol. 8:2074 (2017).

PUBMED DOI

A novel typing method for Streptococcus pneumoniae using selected surface proteins

Domenech A, Moreno J, Ardanuy C, Liñares J, de la Campa AG, Martin-Galiano AJ. Front Microbiol. 2016; 31;7:420.

PUBMED DOI

An increase in negative supercoiling in bacteria reveals topology-reacting gene clusters and a homeostatic response mediated by the DNA topoisomerase I gene

Ferrándiz MJ, Martín-Galiano AJ, Arnanz C, Camacho-Soguero I, Tirado-Vélez JM, de la Campa AG. 2016. Nucl Acids Res. 44:7292-7303 (2016).

PUBMED DOI

Reactive oxygen species contribute to the bactericidal effects of the fluoroquinolone moxifloxacin in Streptococcus pneumoniae

Ferrándiz MJ, Martín-Galiano AJ, Arnanz C, Zimmerman T, de la Campa AG. Antimicrob Agents Chemother. 60:409-417 (2016).

PUBMED DOI

The fluoroquinolone levofloxacin triggers the transcriptional activation of iron transport genes that contribute to cell death in Streptococcus pneumoniae.

Ferrándiz MJ, de la Campa AG. Antimicrob Agents Chemother. 58:247-257 (2014)

PUBMED DOI

Fluoroquinolone-resistant pneumococci: dynamics of serotypes and clones in Spain in 2012 compared with those from 2002 and 2006

Domenech A, Tirado-Vélez JM, Fenoll A, Ardanuy C, Yuste J, Liñares J, de la Campa AG. Antimicrob Agents Chemother. 58:2393-2399 (2014).

PUBMED DOI

The balance between gyrase and topoisomerase I activities determines levels of supercoiling, nucleoid compaction, and viability in bacteria

García-López M, Megias D, Ferrándiz MJ, de la Campa AG. Front Microbiol. 2023; 11;1094692.

PUBMED DOI

Tyrosine kinase 2 modulates splenic B cells through type I IFN and TLR7 signaling.

Bodega-Mayor I, Delgado-Wicke P, Arrabal A, Alegría-Carrasco E, Nicolao-Gómez A, Jaén-Castaño M, Espadas C, Dopazo A, Martín-Gayo E, Gaspar ML, de Andrés B, Fernández-Ruiz E. Cell Mol Life Sci. 2024 Apr 29;81(1):199.

PUBMED DOI

Content with Investigacion Unidad de Genómica Funcional (Tumores Sólidos Infantiles) .

Additional Information

Our group is interested in the mechanisms of generation of hematopoietic cells throughout ontogeny and the influence that the first hematopoietic cells, belonging to the innate/pseudoinnate immune system, exert on the adaptive immune system in the adult individual. Our lines of research include the analysis of pseudoinnate lymphoid populations, which connect the innate and adaptive immune systems, and whose origin is in progenitors of early ontogeny. We are trying to understand its role in the immune response through TLR receptors (“Toll-like receptors”) throughout life, particularly in animal models of bacterial infection, with the aim of designing new vaccine therapies. We analyze immune diversity in animal models and in human samples of children and elderly people diagnosed with respiratory disease, by: a) analyzing the lymphoid subpopulations of the local response. b) the study of the genetic rearrangements of the immunoglobulins that are produced. c) the systemic effector response and in peripheral organs. These approaches, carried out both in the mouse model and in human samples, will facilitate the design of more effective therapies and the characterization of biomarkers, adapted to the pediatric and elderly population, useful for the health field and technological innovation.rupo está interesado en los mecanismos de generación de células hematopoyéticas a lo largo de la ontogenia y la influencia que las primeras células hematopoyéticas, pertenecientes al sistema inmune innato/pseudoinnato, ejercen sobre el sistema inmune adaptativo en el individuo adulto. Nuestras líneas de investigación incluyen el análisis de poblaciones linfoides pseudoinnatas, que conectan el sistema inmune innato y adaptativo, y cuyo origen está en progenitores de la ontogenia temprana. Intentamos entender su papel en la respuesta inmune a través de receptores TLR (“Toll-like receptors”) a lo largo de la vida, en particular en modelos animales de infección bacteriana, con objeto de diseñar nuevas terapias vacunales. Analizamos la diversidad inmune en modelos animales y en muestras humanas de niños y ancianos diagnosticados de enfermedad respiratoria, mediante: a) el análisis de las subpoblaciones linfoides de la respuesta local. b) el estudio de los reordenamientos genéticos de las inmunoglobulinas que se producen. c) la respuesta efectora sistémica y en órganos periféricos. Estas aproximaciones, realizadas tanto en el modelo de ratón como en muestras humanas, facilitarán el diseño de terapias más eficaces y la caracterización de biomarcadores, adaptados a la población infantil y envejecida, de utilidad para el ámbito sanitario y de innovación tecnológica.

Doctoral theses

“Expression and functionality of tlr2 and tlr4 in lymphomyeloid populations present in the lung and lymphoid organs during embryonic and neonatal life in mouse models.”  Carolina Ruiz Sánchez. Complutense University of Madrid, 2022

Master's thesis

Detection and characterization of extracellular vesicles of platelet origin in lung supernatants from SPN-infected animals. Marta Paris, Alcalá University, 2024

Study of TLR-dependent activation in the RAW 264.7 macrophage line. Iñigo Merino de Saracho, Alcalá University, 2023

Study of the functionality of TLR receptors in the lung and other lymphoid organs in B cell populations using the neonatal mouse model. Yolanda Campanero, Alcalá University, 2023

Exploratory study of T lymphoid progenitors in the neonatal mouse lung. Alejandro Arrabal, Complutense University of Madrid, 2022

Study of B lymphoid differentiation in mice deficient for CD5 and CD6 molecules. Cristina Martín, Alcalá University, 2022

Role of platelets and their progenitor cells in two animal models of infection: SPN and RSV. Ana de Lucas Rius, Alcalá University, 2020

Pilot study of RSV infection in the mouse model: cellular phenotype of myeloid and lymphoid populations in the lung in two animal models of infection: SPN and RSV. Juan Antonio Martín Quesada, Alcalá University, 2020

B cell response during Streptococcus pneumoniae infection. Eva Castro, 2020

Study of the hematopoietic potential of neonatal lung cells in the mouse model. Ana Cogollo García, Alcalá University, 2018

Innate immune response to S. pneumoniae in the lung. Rodrigo Sánchez, Complutense University of Madrid, 2018

Neonatal immunity in the mouse model: localization and function of the innate and adaptive response. Alba Ezequiel Fernández, Alcalá University, 2017

Characterization of immunoglobulin gene diversity in the mouse model of TLR4 homeostasis and activation by bacterial lipopolysaccharide. Cristina García Caballero, Alcalá University, 2017

Altered lymphopoiesis and splenic B cell subsets on Telomerase Activity Deficient Mice (TERC-/-). Juliana Manosalva, Complutense University of Madrid, 2017

Study of the Immune Response in Nasopharyngeal Washings of Infants with Bronchiolitis. Isabel Martín Barrios, Complutense University of Madrid, 2016

Dynamics of B1-REL lymphocytes in the in vivo immunization model with DNP-LPS. Inmaculada Sanz Ramos University Alcalá, 2015

Megakaryocyte differentiation pathways in the mouse model. Marta Cobos Briz, Alcalá University, 2015

Role of megakaryocytes in infectious processes. Melania Guerrero Hue, Complutense University of Madrid, 2015

 

Final degree projects

Study of new bacterial vaccines in the mouse (Mus musculus) infection model due to Streptococcus pneumoniae. Alejandro Arrabal, Polytechnic University of Madrid, 2021

Megakaryocytes and platelets in SPN respiratory infection: Role of TLR4. Óscar González Hervás, Complutense University of Madrid, 2021

Study of the immune response mediated by pseudo-innate B lymphocytes against TLR4-dependent immunization models. Rodrigo Sánchez, Complutense University of Madrid, 2017.

Study of the diversity in the immunoglobulin repertoire in healthy individuals. Isabel Martín, Francisco de Vitoria University, 2015.

Dynamics of hematopoietic populations in the perinatal spleen. Inmaculada Sanz, Alcalá University, 2014

Teaching in training courses

Training course: Introduction to Flow Cytometry (from 2015 to present)
Training Course: Flow Cytometry Data Analysis (2018 to present)

Outreach / Citizen Science

• Collaboration in the 4th+Company CAM program.

• Collaboration with the ISCIII Scientific Culture Unit in Science Week at the ISCIII

• Scientific Dissemination Project "Talking about Science", carried out in Majadahonda primary, secondary and high school schools, since 2015 in collaboration with the Department of Education and Youth of the Majadahonda City Council: “How your Immune System works and healthy lifestyle habits to take care of it”

Doctoral theses

“Expression and functionality of tlr2 and tlr4 in lymphomyeloid populations present in the lung and lymphoid organs during embryonic and neonatal life in mouse models.”  Carolina Ruiz Sánchez. Complutense University of Madrid, 2022

Master's thesis

Detection and characterization of extracellular vesicles of platelet origin in lung supernatants from SPN-infected animals. Marta Paris, Alcalá University, 2024

Study of TLR-dependent activation in the RAW 264.7 macrophage line. Iñigo Merino de Saracho, Alcalá University, 2023

Study of the functionality of TLR receptors in the lung and other lymphoid organs in B cell populations using the neonatal mouse model. Yolanda Campanero, Alcalá University, 2023

Exploratory study of T lymphoid progenitors in the neonatal mouse lung. Alejandro Arrabal, Complutense University of Madrid, 2022

Study of B lymphoid differentiation in mice deficient for CD5 and CD6 molecules. Cristina Martín, Alcalá University, 2022

Role of platelets and their progenitor cells in two animal models of infection: SPN and RSV. Ana de Lucas Rius, Alcalá University, 2020

Pilot study of RSV infection in the mouse model: cellular phenotype of myeloid and lymphoid populations in the lung in two animal models of infection: SPN and RSV. Juan Antonio Martín Quesada, Alcalá University, 2020

B cell response during Streptococcus pneumoniae infection. Eva Castro, 2020

Study of the hematopoietic potential of neonatal lung cells in the mouse model. Ana Cogollo García, Alcalá University, 2018

Innate immune response to S. pneumoniae in the lung. Rodrigo Sánchez, Complutense University of Madrid, 2018

Neonatal immunity in the mouse model: localization and function of the innate and adaptive response. Alba Ezequiel Fernández, Alcalá University, 2017

Characterization of immunoglobulin gene diversity in the mouse model of TLR4 homeostasis and activation by bacterial lipopolysaccharide. Cristina García Caballero, Alcalá University, 2017

Altered lymphopoiesis and splenic B cell subsets on Telomerase Activity Deficient Mice (TERC-/-). Juliana Manosalva, Complutense University of Madrid, 2017

Study of the Immune Response in Nasopharyngeal Washings of Infants with Bronchiolitis. Isabel Martín Barrios, Complutense University of Madrid, 2016

Dynamics of B1-REL lymphocytes in the in vivo immunization model with DNP-LPS. Inmaculada Sanz Ramos University Alcalá, 2015

Megakaryocyte differentiation pathways in the mouse model. Marta Cobos Briz, Alcalá University, 2015

Role of megakaryocytes in infectious processes. Melania Guerrero Hue, Complutense University of Madrid, 2015

 

Final degree projects

Study of new bacterial vaccines in the mouse (Mus musculus) infection model due to Streptococcus pneumoniae. Alejandro Arrabal, Polytechnic University of Madrid, 2021

Megakaryocytes and platelets in SPN respiratory infection: Role of TLR4. Óscar González Hervás, Complutense University of Madrid, 2021

Study of the immune response mediated by pseudo-innate B lymphocytes against TLR4-dependent immunization models. Rodrigo Sánchez, Complutense University of Madrid, 2017.

Study of the diversity in the immunoglobulin repertoire in healthy individuals. Isabel Martín, Francisco de Vitoria University, 2015.

Dynamics of hematopoietic populations in the perinatal spleen. Inmaculada Sanz, Alcalá University, 2014

Teaching in training courses

Training course: Introduction to Flow Cytometry (from 2015 to present)
Training Course: Flow Cytometry Data Analysis (2018 to present)

Outreach / Citizen Science

• Collaboration in the 4th+Company CAM program.

• Collaboration with the ISCIII Scientific Culture Unit in Science Week at the ISCIII

• Scientific Dissemination Project "Talking about Science", carried out in Majadahonda primary, secondary and high school schools, since 2015 in collaboration with the Department of Education and Youth of the Majadahonda City Council: “How your Immune System works and healthy lifestyle habits to take care of it”

Content with Investigacion Unidad de Genómica Funcional (Tumores Sólidos Infantiles) .