We protect your health through science
Investigation
Bacterial Genetics
Publications
Staphylococcus aureus Nasal Colonization in Spanish Children. The COSACO Nationwide Surveillance Study.
Staphylococcus aureus Nasal Colonization in Spanish Children. The COSACO Nationwide Surveillance Study. Del Rosal T, Méndez-Echevarría A, Garcia-Vera C, Escosa-Garcia L, Agud M, Chaves F, Román F, Gutierrez-Fernandez J, Ruiz de Gopegui E, Ruiz-Carrascoso G, Ruiz-Gallego MDC, Bernet A, Quevedo SM, Fernández-Verdugo AM, Díez-Sebastian J, Calvo C; COSACO Study Group.
PUBMEDAntimicrobial Resistance and Distribution of Staphylococcus spp. Pulsotypes Isolated from Goat and Sheep Bulk Tank Milk in Southern Spain
Antimicrobial Resistance and Distribution of Staphylococcus spp. Pulsotypes Isolated from Goat and Sheep Bulk Tank Milk in Southern Spain. Barrero-Domínguez B, Luque I, Galán-Relaño Á, Vega-Pla JL, Huerta B, Román F, Astorga RJ. Foodborne Pathog Dis. 2019 Oct;16(10):723-730. doi: 10.1089/fpd.2018.2593. Epub 2019 Jun 3.Foodborne Pathog Dis. 2019. PMID: 31157980
PUBMEDPrevalence of pSCFS7-like vectors among cfr-positive staphylococcal population in Spain
Prevalence of pSCFS7-like vectors among cfr-positive staphylococcal population in Spain. Nguyen LTT*, Román F*, Morikawa K, Trincado P, Marcos C, Rojo-Martín MD, Cafini F. Int J Antimicrob Agents. 2018 Aug;52(2):305-306.
PUBMEDMethodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus.
Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus. Cafini F, Thi Le Thuy N, Román F, Prieto J, Dubrac S, Msadek T, Morikawa K. J Vis Exp. 2017 Mar 10;(121).
PUBMEDContent with Investigacion .
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Isabel Jado García
Científico Titular OPIS, Director laboratorio
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Raquel Escudero Nieto
Científico Titular OPIS, Director laboratorio
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Escolástica Chaparro Tercero
Técnico de Laboratorio
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Ave María Vila-Coro Laviña
Auxiliar de Investigación
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María Elena Andrés Galván
Ayudante de Investigación.
Técnico superior en laboratorio de diagnóstico clínico en 2013 y Técnico superior en laboratorio de análisis y control de calidad en 2015. Lleva vinculada a la Unidad de Neumococos desde 2022 como ayudante de investigación y es personal de plantilla. Anteriormente estuvo contratada en el Instituto de recursos naturales y agrobiología de Salamanca (IRNASA) del CSIC.
List of staff
Additional Information
Streptococcus pneumoniae is a human pathogen that, despite the development of vaccines, continues to be an important cause of mortality and morbidity. We investigate the mechanisms of antibiotic resistance in this bacterium. On the one hand by identifying new therapeutic targets and on the other hand by investigating the molecular basis of the action of antibiotics already used in clinical practice (the fluoroquinolones levofloxacin and moxifloxacin) or not yet used (seconeolitsine). For this purpose, we used a multidisciplinary analysis involving genomics, transcriptomics and proteomics to understand the organization of the S. pneumoniae chromosome and the identification of the factors that stabilize this organization, including ncRNAs. Changes in the level of global supercoiling, either by inhibition of gyrase (decrease) or by inhibition of topoisomerase I (increase) alter the transcriptome. The modulated genes are located in domains, whose genes show specific functional characteristics. The aim is to identify new factors essential for S. pneumoniae physiology and to characterize transcriptional regulation in response to topological stress. In addition, RNA interference technology and CRISPR systems will be used as novel antibacterials. These studies will establish the bases for translational research aimed at the development of new therapeutic targets for the treatment of pneumococcal diseases.
Streptococcus pneumoniae is a human pathogen that, despite the development of vaccines, continues to be an important cause of mortality and morbidity. We investigate the mechanisms of antibiotic resistance in this bacterium. On the one hand by identifying new therapeutic targets and on the other hand by investigating the molecular basis of the action of antibiotics already used in clinical practice (the fluoroquinolones levofloxacin and moxifloxacin) or not yet used (seconeolitsine). For this purpose, we used a multidisciplinary analysis involving genomics, transcriptomics and proteomics to understand the organization of the S. pneumoniae chromosome and the identification of the factors that stabilize this organization, including ncRNAs. Changes in the level of global supercoiling, either by inhibition of gyrase (decrease) or by inhibition of topoisomerase I (increase) alter the transcriptome. The modulated genes are located in domains, whose genes show specific functional characteristics. The aim is to identify new factors essential for S. pneumoniae physiology and to characterize transcriptional regulation in response to topological stress. In addition, RNA interference technology and CRISPR systems will be used as novel antibacterials. These studies will establish the bases for translational research aimed at the development of new therapeutic targets for the treatment of pneumococcal diseases.