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Investigación
Enfermedades bacterianas transmitidas por agua y alimentos
Líneas de investigación
Contenidos con Investigacion .
Legionella
Desde su creación hasta la actualidad, La Unidad de Legionella tiene como principal función dar apoyo científico-técnico a la Administración General del Estado, a las Comunidades Autónomas y al Sistema Nacional de Salud en el campo de la prevención y control de la legionelosis, así como llevar a cabo investigaciones científicas en el contexto de la legionelosis. Además, la Unidad de Legionella también actúa como Laboratorio de Referencia de España frente al European Centre for Disease Prevention and Control (ECDC), siendo miembro de la red europea de vigilancia de la legionelosis, “European Legionnaires’ Disease Surveillance Network (ELDSNet). Finalmente, la unidad también realiza una actividad docente, participando en cursos de formación especializada, así como en Máster Universitarios.
Principales líneas de investigación
Vigilancia microbiológica
Búsqueda de marcadores moleculares con capacidad de predecir el riesgo de una instalación de provocar legionelosis. Factores de virulencia de Legionella spp.
Estudio de la capacidad formadora de biofilms de Legionella spp. Colonización y dispersión.
Búsqueda de marcadores fenotípicos capaces de discriminar especies del Género Legionella; grupos y subgrupos de Legionella pneumophila.
Diferentes estructuras de biofilms en función de la cepa formadora de Legionella pneumophila. En verde la biomasa bacteriana, en rojo el exopolisacárido de la matriz extracelular.
Apoyo al Sistema Nacional de Salud de la Unidad de Legionella
La Unidad de Legionella tambien desarrolla actividades con el fin de proporcionar asistencia al sistema nacional de salud a traves de la oferta disponible en la cartera de servicios del CNM, así como a través de programas de vigilancia microbiológica.
Publicaciones destacadas
A Genome-to-Genome Analysis of Associations between Human Genetic Variation, HIV-1 Sequence Diversity, and Retroviral Control.
Istvan Bartha Jonathan M Carlson, Chanson J Brumme, Paul J McLaren, Zabrina L Brumme, Mina John, David W Haas, Javier Martinez-Picado, Cecilio López Galíndez, Andri Rauch, Huldrych F Günthard, Enos Bernasconi, Pietro Vernazza, Thomas Klimkait, Sabine Yerly, Jennifer Listgarten, Nico Pfeifer, Zoltan Kutalik, Todd M Allen, Viktor Müller, P Richard Harrigan, David Heckerman, Amalio Telenti, and Jacques Fellay, for the HIV Genome-to-Genome Study and the Swiss HIV Cohort Study. A Genome-to-Genome Analysis of Associations between Human Genetic Variation, HIV-1 Sequence Diversity, and Retroviral Control. (2013). Elife. 2013;2:e01123.
PUBMED DOIPrevalence of HIV-1 dual infection in LTNP-Elite Controllers
María Pernas, Concepción Casado, Virginia Sandonis, Carolina Arcones, Carmen Rodríguez , Ezequiel Ruiz-Mateos , Eva Ramírez de Arellano , Norma Rallón , Margarita Del Val , Eulalia Grau, Mariola López-Vazquez , Manuel Leal , Jorge del Romero , Cecilio López Galíndez . Prevalence of HIV-1 dual infection in LTNP-Elite Controllers. (2013). J.of AIDS. 64, 3, 225-231. IF 4.262
PUBMED DOIEvidence of Ongoing Replication in a Human Immunodeficiency Virus Type 1 Persistently Infected Cell Line
Isabel Olivares, Carmen Sánchez-Jiménez, Catarina Reis Vieira, Víctor Toledano, Mónica Gutiérrez-Rivas, and Cecilio López-Galíndez. Evidence of Ongoing Replication in a Human Immunodeficiency Virus Type 1 Persistently Infected Cell Line. (2013). J. of General Virology. 94, 944-954.
PUBMED DOIPrevalence of pSCFS7-like vectors among cfr-positive staphylococcal population in Spain.
Prevalence of pSCFS7-like vectors among cfr-positive staphylococcal population in Spain. Nguyen LTT*, Román F*, Morikawa K, Trincado P, Marcos C, Rojo-Martín MD, Cafini F. Int J Antimicrob Agents. 2018 Aug;52(2):305-306.
PUBMED DOICarbapenemase-Producing Klebsiella pneumoniae From Transplanted Patients in Brazil: Phylogeny, Resistome, Virulome and Mobile Genetic Elements Harboring blaKPC2 or blaNDM-1
Raro OHF, da Silva RMC, Filho EMR, Sukiennik TCT, Stadnik C, Dias CAG, Oteo, Iglesias J, Pérez-Vázquez M. Carbapenemase-Producing Klebsiella pneumoniae From Transplanted Patients in Brazil: Phylogeny, Resistome, Virulome and Mobile Genetic Elements Harboring blaKPC2 or blaNDM-1.Front Microbiol. 2020 jul 15;11:1563.
PUBMED DOICarbapenemase-producing Pseudomonas aeruginosa in Spain: interregional dissemination of the high risk-clones ST175 and ST244 carrying blaVIM-2, blaVIM-1, blaIMP-8, blaVIM-20 and blaKPC-2
Pérez-Vázquez M, Sola-Campoy PJ, Zurita ÁM, et al. Carbapenemase-producing Pseudomonas aeruginosa in Spain: interregional dissemination of the high risk-clones ST175 and ST244 carrying blaVIM-2, blaVIM-1, blaIMP-8, blaVIM-20 and blaKPC-2.Int J Antimicrob Agents. 2020;106026.
PUBMED DOIProspective multicenter study of carbapenemase producing Enterobacteriaceae from 83 hospitals in Spain: High in vitro susceptibility to colistin and meropenem
Oteo J*, Ortega A, Bartolomé R, Bou G, Conejo C, Fernández-Martínez M, González-López JJ, Martínez-García L, Martínez-Martínez L, Merino M, Miró E, Mora M, Navarro F, Oliver A, Pascual Á, Rodríguez-Baño J, Ruiz-Carrascoso G, Ruiz-Garbajosa P, Zamorano L, Bautista V, Pérez-Vázquez M, Campos J; GEIH-GEMARA (SEIMC) and REIPI. Prospective multicenter study of carbapenemase producing Enterobacteriaceae from 83 hospitals in Spain: High in vitro susceptibility to colistin and meropenem. Antimicrob Agents Chemother. 2015; 59(6): 3406-12.
PUBMED DOIEmergence of NDM-producing Klebsiella pneumoniae and Escherichia coli in Spain: phylogeny, resistome, virulence and plasmids encoding blaNDM-like genes as determined by WGS
Pérez-Vázquez M, Sola Campoy PJ, Ortega A, Bautista V, Monzón S, Ruiz-Carrascoso G, Mingorance J, González-Barberá EM, Gimeno C, Aracil B, Sáez D, Lara N, Fernández S, González-López JJ, Campos J, Kingsley RA, Dougan G,Oteo-Iglesias J; Spanish NDM Study Group. Emergence of NDM-producing Klebsiella pneumoniae and Escherichia coli in Spain: phylogeny, resistome, virulence and plasmids encoding blaNDM-like genes as determined by WGS. J Antimicrob Chemother. 2019 Dec 1; A 74(12):3489-3496.
PUBMED DOIZoonotic pathogens in fluctuating common vole (Microtus arvalis) populations: occurrence and dynamics
Rodriguez-Pastor, Ruth; Escudero, Raquel; Lambin, Xavier; Vidal, M Dolors; Gil, Horacio; Jado, Isabel; Rodriguez-Vargas, Manuela; Luque-Larena, Juan Jose; Mougeot, Francois. Zoonotic pathogens in fluctuating common vole (Microtus arvalis) populations: occurrence and dynamics. Parasitology. pp. 1 - 10. 24/09/2018.
PUBMED DOILong-range dispersal moved Francisella tularensis into Western Europe from the East
Dwibedi, Chinmay; Birdsell, Dawn; Larkeryd, Adrian; Myrtennas, Kerstin; Ohrman, Caroline; Nilsson, Elin; Karlsson, Edvin; Hochhalter, Christian; Rivera, Andrew; Maltinsky, Sara; Bayer, Brittany; Keim, Paul; Scholz, Holger C; Tomaso, Herbert; Wittwer, Matthias; Beuret, Christian; Schuerch, Nadia; Pilo, Paola; Hernandez Perez, Marta; Rodriguez-Lazaro, David; Escudero, Raquel; Anda, Pedro; Forsman, Mats; Wagner, David M; Larsson, Par; Johansson, Anders. Long-range dispersal moved Francisella tularensis into Western Europe from the East. Microbial genomics. 2 - 12, pp. e000100. 01/01/2016.
PUBMED DOIFrancisella species in ticks and animals, Iberian Peninsula
Lopes de Carvalho, I.; Toledo, A.; Carvalho, C. L.; Barandika, J. F.; Respicio-Kingry, L. B.; Garcia-Amil, C.; Garcia-Perez, A. L.; Olmeda, A. S.; Ze-Ze, L.; Petersen, J. M.; Anda, P.; Nuncio, M. S.; Escudero, R. Francisella species in ticks and animals, Iberian Peninsula. Ticks and Tick-Borne Diseases. 7 - 1, pp. 159 - 165. Elsevier GMBH, Urban & Fischer Verlag, 01/01/2016.
PUBMED DOIStable levels of Coxiella burnetii prevalence in dairy sheep flocks but changes in genotype distribution after a 10-year period in northern Spain
Álvarez-Alonso R, Barandika JF, Ruiz-Fons F, Ortega-Araiztegi I, Jado I, Hurtado A, García-Pérez AL. Stable levels of Coxiella burnetii prevalence in dairy sheep flocks but changes in genotype distribution after a 10-year period in northern Spain. Acta Vet Scand. 2018 Nov 20;60(1):75.
PUBMED DOIEvidence for Suppression of Onchocerciasis Transmission in Bioko Island, Equatorial Guinea
Moya L, Herrador Z, Ta-Tang TH, Rubio JM, Perteguer MJ, Hernandez-González A, García B, Nguema R, Nguema J, Ncog P, Garate T, Benito A, Sima A and Aparicio P. Evidence for Suppression of Onchocerciasis Transmission in Bioko Island, Equatorial Guinea.PLoS Negl Trop Dis, 2016; 10(7): e0004829.
PUBMED DOILAMP kit for diagnosis of non-falciparum malaria in Plasmodium ovale infected patients
Cuadros J, Martin Ramírez A, González IJ, Ding XC, Perez Tanoira R, Rojo-Marcos G, Gómez-Herruz P, Rubio JM. LAMP kit for diagnosis of non-falciparum malaria in Plasmodium ovale infected patients. Malar J. 2017 Jan 7;16(1):20.
PUBMED DOIPlasmodium species differentiation by non-expert on-line volunteers for remote malaria field diagnosis
Ortiz-Ruiz A, Postigo M, Gil-Casanova S, Cuadrado D, Bautista JM, Rubio JM, Luengo-Oroz M, Linares M. Plasmodium species differentiation by non-expert on-line volunteers for remote malaria field diagnosis. Malar J. 2018 Jan 30;17(1):54.
PUBMED DOIStudy of the diagnostic accuracy of microbiological techniques in the diagnosis of malaria in the immigrant population in Madrid
Martín-Díaz A, Rubio JM, Herrero-Martínez JM, Lizasoain M, Ruiz-Giardin JM, Jaqueti J, Cuadros J, Rojo-Marcos G, Martín-Rabadán P, Calderón M, Campelo C, Velasco M, Pérez-Ayala A. Study of the diagnostic accuracy of microbiological techniques in the diagnosis of malaria in the immigrant population in Madrid. Malar J. 2018 Aug 29;17(1):314.
PUBMED DOIpective comparative multi-centre study on imported Plasmodium ovale wallikeri and Plasmodium ovale curtisi infections.
Rojo-Marcos G, Rubio-Muñoz JM, Angheben A, Jaureguiberry S, García-Bujalance S, Tomasoni LR, Rodríguez-Valero N, Ruiz-Giardín JM, Salas-Coronas J, Cuadros-González J, García-Rodríguez M, Molina-Romero I, López-Vélez R, Gobbi F, Calderón-Moreno M, Martin-Echevarría E, Elía-López M, Llovo-Taboada J; TropNet Plasmodium ovale investigator group. Prospective comparative multi-centre study on imported Plasmodium ovale wallikeri and Plasmodium ovale curtisi infections. Malar J. 2018 Oct 30;17(1):399.
PUBMED DOIImported and autochthonous malaria in West Saudi Arabia: results from a reference hospital
Soliman RH, Garcia-Aranda P, Elzagawy SM, Hussein BE, Mayah WW, Martin Ramirez A, Ta-Tang TH, Rubio JM. Imported and autochthonous malaria in West Saudi Arabia: results from a reference hospital. Malar J. 2018 Aug 7;17(1):286.
PUBMED DOICryptosporidium hominis genotypes involved in increased incidence and clusters of cases, Navarra, Spain, 2012.
Fuentes, I., Martín, C., Beristain, X; Mazón,A, Saugar, JM, Blanco, A; García M, Cenoz, Valle-Cristia, Ezpeleta, C., Castilla, J. 2015. Cryptosporidium hominis genotypes involved in increased incidence and clusters of cases, Navarra, Spain, 2012. Epidemiology and Infection; 143:1033-6
PUBMED DOIMolecular genotyping of Giardia duodenalis isolates from symptomatic individuals attending two major public hospitals in Madrid, Spain.
Lucio A, Martínez-Ruiz R, Merino FJ, Bailo B, Aguilera M, Fuentes I, Carmena D. 2015. Molecular genotyping of Giardia duodenalis isolates from symptomatic individuals attending two major public hospitals in Madrid, Spain. PLoS One. 10 (12): e0143981.
PUBMED DOIOccurrence and subtype distribution of Blastocystis sp. in humans, dogs, and cats sharing household in northern Spain and assessment of zoonotic transmission risk.
Paulos S, Köster PC, de Lucio A, Hernández-de-Mingo M, Cardona GA, Fernández-Crespo JC, Stensvold RC, Carmena D. 2018. Occurrence and subtype distribution of Blastocystis sp. in humans, dogs, and cats sharing household in northern Spain and assessment of zoonotic transmission risk. Zoonoses and Public Health, 65:993-1002.
PUBMED DOIPhlebotomine sand fly survey in the focus of leishmaniasis of Madrid, Spain (2012–2014): seasonal dynamics, Leishmania infantum infection rates and blood meal preferences.
González E, Jiménez M, Hernández S, Martín-Martín I, Molina R. Phlebotomine sand fly survey in the focus of leishmaniasis of Madrid, Spain (2012–2014): seasonal dynamics, Leishmania infantum infection rates and blood meal preferences. Parasit Vectors 2017, 10:368.
PUBMED DOIMethods in Sand Fly Research
Molina R, Jiménez M, Alvar J, González E, Hernández-Taberna S, Martín-Martín Inés. 2017. Methods in Sand Fly Research (R. Molina, M. Jiménez & J. Alvar, edits.). Servicio de Publicaciones Universidad de Alcalá de Henares. ISBN: 978-84-16978-28-1
Factors associated with Leishmania asymptomatic infection: results from a cross-sectional survey in highland northern Ethiopia
Custodio E, Gadisa E, Sordo L, Cruz I, Moreno J, Nieto J, Chicharro C, Aseffa A, Abraham Z, Hailu T, Cañavate C. Factors associated with Leishmania asymptomatic infection: results from a cross-sectional survey in highland northern Ethiopia. PLoS Negl Trop Dis. 2012;6(9):e1813.
PUBMED DOICytokine Release Assays as Tests for Exposure to Leishmania, and for Confirming Cure from Leishmaniasis, in Solid Organ Transplant Recipients.
Carrillo E, Carrasco-Antón N, López-Medrano F, Salto E, Fernández L, San Martín JV, Alvar J, Aguado JM, Moreno J. Cytokine Release Assays as Tests for Exposure to Leishmania, and for Confirming Cure from Leishmaniasis, in Solid Organ Transplant Recipients. PLoS Negl Trop Dis. 2015 Oct 23;9(10):e0004179.
PUBMED DOIChemotactic Protein 1 in Plasma from Soluble Leishmania Antigen-Stimulated Whole Blood as a Potential Biomarker of the Cellular Immune Response to Leishmania infantum
Ibarra-Meneses AV, Sanchez C, Alvar J, Moreno J, Carrillo E. Monocyte Chemotactic Protein 1 in Plasma from Soluble Leishmania Antigen-Stimulated Whole Blood as a Potential Biomarker of the Cellular Immune Response to Leishmania infantum. Front Immunol. 2017 Sep 29;8:1208.
PUBMED DOICytokines and chemokines measured in dried SLA-stimulated whole blood spots for asymptomatic Leishmania infantum and Leishmania donovani infection.
Ibarra-Meneses AV, Mondal D, Alvar J, Moreno J, Carrillo E. Cytokines and chemokines measured in dried SLA-stimulated whole blood spots for asymptomatic Leishmania infantum and Leishmania donovani infection. Sci Rep. 2017 Dec 8;7(1):17266.
PUBMED DOICellular Markers of Active Disease and Cure in Different Forms of Leishmania infantum-Induced Disease.
Botana L, Matía B, San Martin JV, Romero-Maté A, Castro A, Molina L, Fernandez L, Ibarra-Meneses A, Aguado M, Sánchez C, Horrillo L, Chicharro C, Nieto J, Ortega S, Ruiz-Giardin JM, Carrillo E, Moreno J. Cellular Markers of Active Disease and Cure in Different Forms of Leishmania infantum-Induced Disease. Front Cell Infect Microbiol. 2018 Nov 13;8:381.
PUBMED DOICarroll MW et al. Temporal and spatial analysis of the 2014-2015 Ebola virus outbreak in West Africa. Nature.
Carroll MW et al. Temporal and spatial analysis of the 2014-2015 Ebola virus outbreak in West Africa. Nature. 2015 Aug 6;524(7563):97-101. doi: 10.1038/nature14594. Epub 2015 Jun 17. PMID: 26083749.
Fernandez-Garcia MD, Meertens L, Chazal M, Hafirassou ML, Dejarnac O, Zamborlini A, Despres P, Sauvonnet N, Arenzana-Seisdedos F, Jouvenet N, Amara A. Vaccine and Wild-Type Strains of Yellow Fever Virus Engage Distinct Entry Mechanisms and Differentially Stimulate Antiviral Immune Responses.
Fernandez-Garcia MD, Meertens L, Chazal M, Hafirassou ML, Dejarnac O, Zamborlini A, Despres P, Sauvonnet N, Arenzana-Seisdedos F, Jouvenet N, Amara A. Vaccine and Wild-Type Strains of Yellow Fever Virus Engage Distinct Entry Mechanisms and Differentially Stimulate Antiviral Immune Responses. mBio. 2016 Feb 9;7(1):e01956-15. doi: 10.1128/mBio.01956-15. PMID: 26861019; PMCID:PMC4752603.
Identification and whole-genome characterization of a recombinant Enterovirus B69 isolated from a patient with Acute Flaccid Paralysis in Niger, 2015
Fernandez-Garcia MD, Majumdar M, Kebe O, Ndiaye K, Martin J. Identification and whole-genome characterization of a recombinant Enterovirus B69 isolated from a patient with Acute Flaccid Paralysis in Niger, 2015. Sci Rep. 2018 Feb 1;8(1):2181. doi: 10.1038/s41598-018-20346-9. PMID: 29391547; PMCID: PMC5795009.
Majumdar M, Sharif S, Klapsa D, Wilton T, Alam MM, Fernandez-Garcia MD, Rehman L, Mujtaba G, McAllister G, Harvala H, Templeton K, Mee ET, Asghar H, Ndiaye K, Minor PD, Martin J. Environmental Surveillance Reveals Complex Enterovirus Circulation Patterns in Human Populations. Open Forum Infect Dis. 2018
Majumdar M, Sharif S, Klapsa D, Wilton T, Alam MM, Fernandez-Garcia MD, Rehman L, Mujtaba G, McAllister G, Harvala H, Templeton K, Mee ET, Asghar H, Ndiaye K, Minor PD, Martin J. Environmental Surveillance Reveals Complex Enterovirus Circulation Patterns in Human Populations. Open Forum Infect Dis. 2018 Oct 1;5(10):ofy250. doi: 10.1093/ofid/ofy250. PMID: 30377626; PMCID: PMC6201154.
Fernandez-Garcia MD, Majumdar M, Kebe O, Fall AD, Kone M, Kande M, Dabo M, Sylla MS, Sompare D, Howard W, Faye O, Martin J, Ndiaye K. Emergence of Vaccine-Derived Polioviruses during Ebola Virus Disease Outbreak, Guinea, 2014-2015.
Fernandez-Garcia MD, Majumdar M, Kebe O, Fall AD, Kone M, Kande M, Dabo M, Sylla MS, Sompare D, Howard W, Faye O, Martin J, Ndiaye K. Emergence of Vaccine-Derived Polioviruses during Ebola Virus Disease Outbreak, Guinea, 2014-2015. Emerg Infect Dis. 2018 Jan;24(1):65-74. doi: 10.3201/eid2401.171174. PMID:29260690; PMCID: PMC5749474.
Tarragó, D.; Mateos, M.-L.; Avellón, A.; Pérez-Vázquez, M.-D.; Tenorio, A.2004
Tarragó, D.; Mateos, M.-L.; Avellón, A.; Pérez-Vázquez, M.-D.; Tenorio, A.2004. Quantitation of Cytomegalovirus DNA in Cerebrospinal Fluid and Serum Specimens from AIDS Patients Using a Novel Highly Sensitive Nested Competitive PCR and the Cobas Amplicor CMV Monitor™ Journal of Medical Virology. 72-2, pp.249-256. ISSN 01466615. 10. Tarragó, D.; Quereda, C.; Tenorio, A.2003. Different cytomegalovirus glycoprotein B genotype distribution in serum and cerebrospinal fluid specimens determined by a novel multiplex nested PCR Journal of Clinical Microbiology. 41-7, pp.2872-2877. ISSN 00951137.
Fernandez-Garcia, Maria Dolores (AC); Kebe, Ousmane; Fall, Aichatou D.; Dia, Hamet; Diop, Ousmane M.; Delpeyroux, Francis; Ndiaye, Kader. 2016.
Fernandez-Garcia, Maria Dolores (AC); Kebe, Ousmane; Fall, Aichatou D.; Dia, Hamet; Diop, Ousmane M.; Delpeyroux, Francis; Ndiaye, Kader. (1/ 7). 2016. Enterovirus A71 Genogroups C and E in Children with Acute Flaccid Paralysis, West Africa EMERGING INFECTIOUS DISEASES. 22-4, pp.753-755. ISSN 1080-6040.
Molecular epidemiology of coxsackievirus B3 infection in Spain, 2004-2015.
K Calderón, M Díaz-de Cerio, C Muñoz-Almagro, N Rabella, I Martínez-Rienda, A Moreno-Docón, G Trallero, M Cabrerizo*. Molecular epidemiology of coxsackievirus B3 infection in Spain, 2004-2015. Arch Virol 161: 1365-1370 (2016).
PUBMED DOIDevelopment and Evaluation of a Serological Assay for the Diagnosis of Tuberculosis in Alpacas and Llamas.
Development and Evaluation of a Serological Assay for the Diagnosis of Tuberculosis in Alpacas and Llamas. Infantes-Lorenzo, Jose A.; Whitehead, Claire E.; Moreno, Inmaculada; et ál..FRONTIERS IN VETERINARY SCIENCE Volumen: 5 Número de artículo: 189 Fecha de publicación: AUG 13 2018
PUBMED DOIInfluence of the Microenvironment in the Transcriptome of Leishmania infantum Promastigotes: Sand Fly versus Culture
Influence of the Microenvironment in the Transcriptome of Leishmania infantum Promastigotes: Sand Fly versus Culture. Alcolea, Pedro J.; Alonso, Ana; Dominguez, Mercedes; et ál..PLOS NEGLECTED TROPICAL DISEASES Volumen: 10 Número: 5 Número de artículo: e0004693 Fecha de publicación: MAY 2016
PUBMED DOIFernandez-Garcia MD, Kebe O, Fall AD, Ndiaye K. Identification and molecular characterization of non-polio enteroviruses from children with acute flaccid paralysis in West Africa, 2013-2014.
Fernandez-Garcia MD, Kebe O, Fall AD, Ndiaye K. Identification and molecular characterization of non-polio enteroviruses from children with acute flaccid paralysis in West Africa, 2013-2014. Scientific Reports. 2017 Jun 19; 7(1):3808. doi: 10.1038/s41598-017-03835-1 ISSN 2045-2322 PMID 28630462
Fernandez-Garcia MD, Manasi Majumdar, Ousmane Kebe, Kader Ndiaye, Javier Martin. Identification and whole-genome characterization of a recombinant Enterovirus B69 isolated from a patient with Acute Flaccid Paralysis in Niger, 2015
Fernandez-Garcia MD, Manasi Majumdar, Ousmane Kebe, Kader Ndiaye, Javier Martin. Identification and whole-genome characterization of a recombinant Enterovirus B69 isolated from a patient with Acute Flaccid Paralysis in Niger, 2015. Scientific Reports. 2018 Feb; 8(1):2181. doi: 10.1038/s41598-018-20346-9. ISSN 2045-2322 PMID 29391547
Fernandez-Garcia MD, Majumdar M, Kebe O, Fall AD, Kone M, Kande M, Dabo M, Sylla MS, Sompare D, Howard W, Faye O, Martin J, Ndiaye K. Emergence of Vaccine- Derived Polioviruses during Ebola Virus Disease Outbreak, Guinea, 2014-2015.
Fernandez-Garcia MD, Majumdar M, Kebe O, Fall AD, Kone M, Kande M, Dabo M, Sylla MS, Sompare D, Howard W, Faye O, Martin J, Ndiaye K. Emergence of Vaccine- Derived Polioviruses during Ebola Virus Disease Outbreak, Guinea, 2014-2015. Emerg Infect Dis. 2018 Jan;24(1):65-74. doi: 10.3201/eid2401.171174. PMID: 29260690; PMCID: PMC5749474.
Cabrerizo M, García-Iñiguez JP, Munell F, Amado A, Madurga-Revilla P, Rodrigo C, Pérez S, Martínez-Sapiña A, Antón A, Suárez G, Rabella N, Del Campo V, Otero A, Masa-Calles J. First Cases of Severe Flaccid Paralysis Associated With Enterovirus D68 Infection in Spain, 2015-2016.
Cabrerizo M, García-Iñiguez JP, Munell F, Amado A, Madurga-Revilla P, Rodrigo C, Pérez S, Martínez-Sapiña A, Antón A, Suárez G, Rabella N, Del Campo V, Otero A, Masa-Calles J. First Cases of Severe Flaccid Paralysis Associated With Enterovirus D68 Infection in Spain, 2015-2016. Pediatr Infect Dis J. 2017 Dec;36(12):1214-1216. doi: 10.1097/INF.0000000000001668. PMID: 28661963.
van Beek J, de Graaf M, Al-Hello H, Allen DJ, Ambert-Balay K, Botteldoorn N, Brytting M, Buesa J, Cabrerizo M, Chan M, Cloak F, Di Bartolo I, Guix S, Hewitt J, Iritani N, Jin M, Johne R, Lederer I, Mans J, Martella V, Maunula L, McAllister G, Niendorf S, Niesters HG, Podkolzin AT, Poljsak-Prijatelj M, Rasmussen LD, Reuter G, Tuite G, Kroneman A, Vennema H, Koopmans MPG; NoroNet. Molecular surveillance of norovirus, 2005-16
van Beek J, de Graaf M, Al-Hello H, Allen DJ, Ambert-Balay K, Botteldoorn N, Brytting M, Buesa J, Cabrerizo M, Chan M, Cloak F, Di Bartolo I, Guix S, Hewitt J, Iritani N, Jin M, Johne R, Lederer I, Mans J, Martella V, Maunula L, McAllister G, Niendorf S, Niesters HG, Podkolzin AT, Poljsak-Prijatelj M, Rasmussen LD, Reuter G, Tuite G, Kroneman A, Vennema H, Koopmans MPG; NoroNet. Molecular surveillance of norovirus, 2005-16: an epidemiological analysis of data collected from the NoroNet network. Lancet Infect Dis. 2018 May;18(5):545-553. doi: 10.1016/S1473-3099(18)30059-8. Epub 2018 Jan 26. PMID: 29396001.
González-Serrano L, Muñoz-Algarra M, González-Sanz R, Portero-Azorín MF, Amaro MJ, Higueras P, Cabrerizo M. Viral gastroenteritis in hospitalized patients: Evaluation of immunochromatographic methods for rapid detection in stool samples. J Clin Virol. 2020 Jul;
González-Serrano L, Muñoz-Algarra M, González-Sanz R, Portero-Azorín MF, Amaro MJ, Higueras P, Cabrerizo M. Viral gastroenteritis in hospitalized patients: Evaluation of immunochromatographic methods for rapid detection in stool samples. J Clin Virol. 2020 Jul; 128:104420. doi: 10.1016/j.jcv.2020.104420. Epub 2020 May 15. PMID: 32454428.
Bubba L, Broberg EK, Jasir A, Simmonds P, Harvala H; Enterovirus study collaborators. Circulation of non-polio enteroviruses in 24 EU and EEA countries between 2015 and 2017
Bubba L, Broberg EK, Jasir A, Simmonds P, Harvala H; Enterovirus study collaborators. Circulation of non-polio enteroviruses in 24 EU and EEA countries between 2015 and 2017: a retrospective surveillance study. Lancet Infect Dis. 2020 Mar;20(3):350-361. doi: 10.1016/S1473-3099(19)30566-3. Epub 2019 Dec 20. PMID: 31870905.
Fernandez-Garcia MD, Simon-Loriere E, Kebe O, Sakuntabhai A, Ndiaye K. Identification and molecular characterization of the first complete genome sequence of Human Parechovirus type 15. Sci Rep. 2020 Apr 21
Fernandez-Garcia MD, Simon-Loriere E, Kebe O, Sakuntabhai A, Ndiaye K. Identification and molecular characterization of the first complete genome sequence of Human Parechovirus type 15. Sci Rep. 2020 Apr 21;10(1):6759. doi: 10.1038/s41598-020-63467-w. PMID: 32317760; PMCID: PMC7174385.
Casas-Alba D, Valero-Rello A, Muchart J, Armangué T, Jordan I, Cabrerizo M, Molero-Luís M, Artuch R, Fortuny C, Muñoz-Almagro C, Launes C. Cerebrospinal Fluid Neopterin in Children With Enterovirus-Related Brainstem Encephalitis. Pediatr Neurol. 2019 Jul
Casas-Alba D, Valero-Rello A, Muchart J, Armangué T, Jordan I, Cabrerizo M, Molero-Luís M, Artuch R, Fortuny C, Muñoz-Almagro C, Launes C. Cerebrospinal Fluid Neopterin in Children With Enterovirus-Related Brainstem Encephalitis. Pediatr Neurol. 2019 Jul; 96:70-73. doi: 10.1016/j.pediatrneurol.2019.01.024. Epub 2019 Feb 7. PMID: 30935719.
González-Sanz R, Taravillo I, Reina J, Navascués A, Moreno-Docón A, Aranzamendi M, Romero MP, Del Cuerpo M, Pérez-González C, Pérez-Castro S, Otero A, Cabrerizo M. Enterovirus D68-associated respiratory and neurological illness in Spain, 2014-2018.
González-Sanz R, Taravillo I, Reina J, Navascués A, Moreno-Docón A, Aranzamendi M, Romero MP, Del Cuerpo M, Pérez-González C, Pérez-Castro S, Otero A, Cabrerizo M. Enterovirus D68-associated respiratory and neurological illness in Spain, 2014-2018. Emerg Microbes Infect. 2019;8(1):1438-1444. doi: 10.1080/22221751.2019.1668243. PMID: 31571527; PMCID: PMC6781473.
Monocytic Myeloid-Derived Suppressor Cells Accumulate in Renal Transplant Patients and Mediate CD4(+) Foxp3(+) Treg Expansion
Luan Y, Mosheir E, Menon MC, Wilson D, Woytovich C, Ochando J, Murphy B. Monocytic Myeloid-Derived Suppressor Cells Accumulate in Renal Transplant Patients and Mediate CD4(+) Foxp3(+) Treg Expansion. 2013. Am J Transplant.13(12):3123-31.
PUBMED DOIRiquelme P, Haarer J, Kammler A, Walter L, Tomiuk S, Ahrens N, Goecze I, Wege A, Fändrich F, Schlitt H, Banas B, Lutz M, Sawitzki B, Ochando J, Geissler E and Hutchinson J. Generation of BTNL8+ TIGIT+ Tregs by Human Regulatory Macrophages Before Kidney Transplantation. Nat Commun.
Riquelme P, Haarer J, Kammler A, Walter L, Tomiuk S, Ahrens N, Goecze I, Wege A, Fändrich F, Schlitt H, Banas B, Lutz M, Sawitzki B, Ochando J, Geissler E and Hutchinson J. Generation of BTNL8+ TIGIT+ Tregs by Human Regulatory Macrophages Before Kidney Transplantation. Nat Commun. 2018; Jul 20;9(1):2858. PMID: 30030423.
Inhibiting Inflammation with Myeloid Cell-Specific Nanobiologics Promotes Organ Transplant Acceptance
Braza MS, Lameijer M, Sanchez-Gaytan B, Arts R, Pérez-Medina C, Conde P, Brahmachary M, van der Touw W, Fay F, Kluza E, Kossatz S, Stroes E, Kroon J, Dress R, Salem F, Rialdi A, Reiner T, Boros P, van Leent M, Strijkers G, Calcagno C, Ginhoux F, Marazzi I, Lutgens E, Nicolaes G, Weber C, Swirski F, Nahrendorf M, Fisher E, Fayad Z, Duivenvoorden R, Netea M, Mulder WJ, and Ochando J. Inhibiting Inflammation with Myeloid Cell-Specific Nanobiologics Promotes Organ Transplant Acceptance.Immunity. 2018; 20;49(5):819-828.e6. PMID: 30413362.
PUBMED DOIFernandez-Garcia MD, Volle R, Joffret ML, Sadeuh-Mba SA, Gouandjika-Vasilache I, Kebe O, Wiley MR, Majumdar M, Simon-Loriere E, Sakuntabhai A, Palacios G, Martin J, Delpeyroux F, Ndiaye K, Bessaud M. Genetic Characterization of Enterovirus A71 Circulating in Africa.
Fernandez-Garcia MD, Volle R, Joffret ML, Sadeuh-Mba SA, Gouandjika-Vasilache I, Kebe O, Wiley MR, Majumdar M, Simon-Loriere E, Sakuntabhai A, Palacios G, Martin J, Delpeyroux F, Ndiaye K, Bessaud M. Genetic Characterization of Enterovirus A71 Circulating in Africa. Emerg Infect Dis. 2018 Apr;24(4):754-757. doi: 10.3201/eid2404.171783. PMID: 29553325; PMCID: PMC5875259.
Leon KE, Schubert RD, Casas-Alba D, Hawes IA, Ramachandran PS, Ramesh A, Pak JE, Wu W, Cheung CK, Crawford ED, Khan LM, Launes C, Sample HA, Zorn KC, Cabrerizo M, Valero-Rello A, Langelier C, Muñoz-Almagro C, DeRisi JL, Wilson MR. Genomic and serologic characterization of enterovirus A71 brainstem encephalitis. Neurol Neuroimmunol Neuroinflamm. 2020
Leon KE, Schubert RD, Casas-Alba D, Hawes IA, Ramachandran PS, Ramesh A, Pak JE, Wu W, Cheung CK, Crawford ED, Khan LM, Launes C, Sample HA, Zorn KC, Cabrerizo M, Valero-Rello A, Langelier C, Muñoz-Almagro C, DeRisi JL, Wilson MR. Genomic and serologic characterization of enterovirus A71 brainstem encephalitis. Neurol Neuroimmunol Neuroinflamm. 2020 Mar 5;7(3):e703. doi: 10.1212/NXI.0000000000000703. PMID: 32139440; PMCID: PMC7136061.
González-Sanz R, Casas-Alba D, Launes C, Muñoz-Almagro C, Ruiz-García MM, Alonso M, González-Abad MJ, Megías G, Rabella N, Del Cuerpo M, Gozalo-Margüello M, González-Praetorius A, Martínez-Sapiña A, Goyanes-Galán MJ, Romero MP, Calvo C, Antón A, Imaz M, Aranzamendi M, Hernández-Rodríguez Á, Moreno-Docón A, Rey- Cao S, Navascués A, Otero A, Cabrerizo M. Molecular epidemiology of an enterovirus A71 outbreak associated with severe neurological disease, Spain, 2016. Euro Surveill. 2019
González-Sanz R, Casas-Alba D, Launes C, Muñoz-Almagro C, Ruiz-García MM, Alonso M, González-Abad MJ, Megías G, Rabella N, Del Cuerpo M, Gozalo-Margüello M, González-Praetorius A, Martínez-Sapiña A, Goyanes-Galán MJ, Romero MP, Calvo C, Antón A, Imaz M, Aranzamendi M, Hernández-Rodríguez Á, Moreno-Docón A, Rey- Cao S, Navascués A, Otero A, Cabrerizo M. Molecular epidemiology of an enterovirus A71 outbreak associated with severe neurological disease, Spain, 2016. Euro Surveill. 2019 Feb;24(7):1800089. doi: 10.2807/1560-7917.ES.2019.24.7.1800089. PMID: 30782267; PMCID: PMC6381658.
Spanish Afp Surveillance Working Group. Acute flaccid paralysis (AFP) surveillance: challenges and opportunities from 18 years' experience, Spain, 1998 to 2015. Euro Surveill.
Spanish Afp Surveillance Working Group. Acute flaccid paralysis (AFP) surveillance: challenges and opportunities from 18 years' experience, Spain, 1998 to 2015. Euro Surveill. 2018 Nov;23(47):1700423. doi: 10.2807/1560-7917.ES.2018.23.47.1700423. PMID: 30482263; PMCID: PMC6341937.
Contenidos con Investigacion .
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Caroline Stephanie Crisóstomo Vergara
Técnico de Laboratorio
Código ORCID: 0009-0008-0525-1737
Técnico de Laboratorio. Técnico superior de Laboratorio Clínico y Biomédico por la Escuela Técnica de Enseñanzas Especializadas de Madrid. Máster en Microbiología Clínica por el Instituto Europeo de Química, Física y Biología de Madrid.
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Laura del Estal Gómez
Ayudante de investigación
Código ORCID: 0009-0000-2773-8986
Graduada en Biología Sanitaria por la Universidad de Alcalá. Máster Universitario en Microbiología Aplicada a la Salud Pública e Investigación en Enfermedades Infecciosas.
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Almudena Cascajero Díaz
Técnico de laboratorio
Código ORCID: 0000-0002-9654-3100
Técnico de laboratorio (Unidad de Inmunopatología del SIDA y Legionella, Centro Nacional de Microbiología).
Técnico superior de laboratorio de diagnóstico clínico por IES Renacimiento de Madrid. Acumula más de 400 horas de formación en técnicas de laboratorio en proteómica, Genómica, Prevención de Riesgos Laborales y Microbiología.
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Juana María González Rubio
Científica Titular
Código ORCID: 0000-0001-6979-2964
La Dra. Juana María González Rubio es Licenciada en Bioquímica por la Universidad de Salamanca y Doctora por la Facultad de Medicina de la Universidad Autónoma de Madrid. Actualmente, es Científico Titular de plantilla en el Centro Nacional de Microbiología del Instituto de Salud Carlos III (ISCIII), donde trabaja en la Unidad de Legionella del Laboratorio de Referencia e Investigación en Enfermedades Bacterianas transmitidas por agua y alimentos.
Dentro del laboratorio, realiza las actividades propias del Programa de Vigilancia Microbiológica de Legionella, y lleva las líneas de investigación del laboratorio sobre la caracterización de biofilms y la puesta a punto de nuevas técnicas para la caracterización de Legionella. También forma parte del equipo investigador del proyecto “Búsqueda de marcadores de patogenicidad para el análisis de riesgos en las instalaciones".
Anteriormente, ha trabajado en la Unidad de Biomonitorización humana del Centro Nacional de Sanidad Ambiental (ISCIII) participando en diferentes proyectos de investigación relacionados con la Sanidad Ambiental, siendo el último más destacado el proyecto “HBM4EU" en el que ha trabajado hasta junio de 2023.
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Fernando González Camacho
Científico Titular
Código ORCID: 0000-0003-3175-9004
Licenciado en Ciencias Biológicas por la Universidad de Salamanca y Doctor por la Universidad Autónoma de Madrid. Actualmente, es Científico Titular de plantilla en el Centro Nacional de Microbiología (CNM) del Instituto de Salud Carlos III (ISCIII). Responsable de la Unidad de Legionella del Laboratorio de Referencia e Investigación en Enfermedades Bacterianas transmitidas por agua y alimentos.
A nivel europeo es sustituto (Alternate) al National Focal Point para la enfermedad del legionario en el ECDC y es OCP (Operational Contact Point) en microbiología para la legionelosis en la European Legionnaires' Disease Surveillance Network (ELDSNet).
Coordina las líneas de investigación del laboratorio que se desarrollan en tres perspectivas diferentes: en las instalaciones colonizadas, estudios sobre la resistencia a los tratamientos y su persistencia; en la clínica, sobre factores de virulencia y su interacción con el sistema inmune; y en la vigilancia microbiológica, sobre la mejorar de los métodos de caracterización del microorganismo.
Es Investigador Principal en el proyecto “Búsqueda de biomarcadores de patogenicidad en Legionella spp con interés predictivo de riesgo de infección".
Es miembro de distintas sociedades científicas como son la Sociedad Española de Salud Ambiental (SESA), Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica (SEIMC) y la European Society of Clinical Microbiology and Infectious Diseases (ESCMID).
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